Joint International Research Laboratory of Animal Health and Food Safety of Ministry of Education & Single Molecule Nanometry Laboratory (Sinmolab), Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China.
School of Manufacture Science and Engineering, Key Laboratory of Testing Technology for Manufacturing Process, Ministry of Education, Southwest University of Science and Technology, Mianyang, Sichuan, 621010, China.
Anal Chim Acta. 2021 Jun 15;1164:338524. doi: 10.1016/j.aca.2021.338524. Epub 2021 Apr 16.
Avian influenza virus (AIV) is a serious zoonotic disease causing severe damages to both poultry industry and human health. To rapidly detect AIV on-site with high sensitivity and accuracy, we design sensitive antibody fluorescence immunosorbent assay (SAFIA) on AIV H9N2 antibody. In SAFIA, hemagglutinin (HA1) protein coated sample chamber specifically binds targets but remarkably reduces non-specific absorption; Protein L coated polystyrene microsphere captures target through secondary antibody to significantly amplify the fluorescence signal; and a portable fluorescence counter automatically measures the fluorescence spot density for AIV H9N2 antibody detection. Proved by practical applications, SAFIA could probe AIV H9N2 antibody in high sensitivity and selectivity, and distinguish positive and negative serum samples in high accuracy. Additionally, SAFIA can rapidly detect AIV H9N2 antibody at room temperature only requiring simple operations as well as cost-effective and compact devices. Therefore, SAFIA is a potential new-generation tool in rapid on-site testing for agricultures.
禽流感病毒（AIV）是一种严重的人畜共患病，对家禽业和人类健康造成严重损害。为了快速、灵敏、准确地现场检测 AIV，我们针对 AIV H9N2 抗体设计了敏感抗体荧光免疫吸附测定法（SAFIA）。在 SAFIA 中，血凝素（HA1）蛋白包被的样本腔特异性结合靶标，但显著减少非特异性吸附；包被蛋白 L 的聚苯乙烯微球通过二级抗体捕获靶标，从而显著放大荧光信号；便携式荧光计数器自动测量 AIV H9N2 抗体检测的荧光斑点密度。实际应用证明，SAFIA 能够以高灵敏度和选择性探测 AIV H9N2 抗体，以高精度区分阳性和阴性血清样本。此外，SAFIA 可以在室温下快速检测 AIV H9N2 抗体，仅需简单操作，且设备具有成本效益、体积小巧。因此，SAFIA 是农业领域快速现场检测的新一代潜在工具。
