Sensing System Research Center, The National Institute of Advanced Industrial Science and Technology, 07-1 Shuku-Machi, Tosu 841-0052, Japan.
Biosensors (Basel). 2021 Jul 26;11(8):250. doi: 10.3390/bios11080250.
The global damage that a widespread viral infection can cause is evident from the ongoing COVID-19 pandemic. The importance of virus detection to prevent the spread of viruses has been reaffirmed by the pandemic and the associated social and economic damage. Surface plasmon resonance (SPR) in microscale and localized SPR (LSPR) in nanoscale virus sensing systems are thought to be useful as next-generation detection methods. Many studies have been conducted on ultra-sensitive technologies, especially those based on signal amplification. In some cases, it has been reported that even a low viral load can be measured, indicating that the virus can be detected in patients even in the early stages of the viral infection. These findings corroborate that SPR and LSPR are effective in minimizing false-positives and false-negatives that are prevalent in the existing virus detection techniques. In this review, the methods and signal responses of SPR and LSPR-based virus detection technologies are summarized. Furthermore, this review surveys some of the recent developments reported and discusses the limitations of SPR and LSPR-based virus detection as the next-generation detection technologies.
从当前的 COVID-19 大流行可以明显看出，广泛的病毒感染会造成全球性的损害。病毒检测对于防止病毒传播的重要性已经在疫情中得到了再次确认，以及与之相关的社会和经济损失。在微尺度的表面等离子体共振（SPR）和纳米尺度的局域表面等离子体共振（LSPR）病毒传感系统中，人们认为它们是下一代检测方法的有用工具。已经有许多关于超灵敏技术的研究，特别是基于信号放大的技术。在某些情况下，据报道，即使是低病毒载量也可以被测量，这表明即使在病毒感染的早期阶段，也可以在患者中检测到病毒。这些发现证实了 SPR 和 LSPR 可以有效地最小化现有病毒检测技术中普遍存在的假阳性和假阴性。在这篇综述中，总结了基于 SPR 和 LSPR 的病毒检测技术的方法和信号响应。此外，本文还调查了一些最近报道的进展，并讨论了 SPR 和 LSPR 基病毒检测作为下一代检测技术的局限性。
