Faculty of Agriculture and Food, Yunnan Institute of Food Safety, Kunming University of Science and Technology, Kunming, Yunnan Province 650500, People's Republic of China.
Food Res Int. 2021 May;143:110264. doi: 10.1016/j.foodres.2021.110264. Epub 2021 Mar 7.
The chemical constituents and antioxidant activity of the three different extracts (80% methanol, 80% ethanol, and 80% acetone) of Crateva unilocalaris Buch. shoots were investigated. Six phenolic compounds and seven saponins were characterized in all extracts. Chikusetsusaponin IVa had the highest content (17.92 to 29.16 mg/g), and chlorogenic acid was the most abundant phenolic compound (10.48 to 13.99 mg/g). The acetone extract had the highest total phenolic, flavonoid, and saponin contents and the strongest antioxidant activity. Moreover, all extracts exhibited good effects on the inhibition of intracellular ROS generation in HepG2 cells. Phenolic compounds but not saponins contributed significantly to the DPPH or ABTS radical scavenging activity of C. unilocalaris shoots according to the results of DPPH•-UHPLC-HRMS and ABTS•+-UHPLC-HRMS analyses. These results may be helpful for further understandings and utilization of C. unilocalaris shoots as a potential natural source in the food or nutraceuticals industry.
对三叶崖爬藤茎的 3 种不同提取物（80%甲醇、80%乙醇和 80%丙酮）的化学成分和抗氧化活性进行了研究。在所有提取物中均鉴定出 6 种酚类化合物和 7 种皂苷。柴胡皂苷 IVa 的含量最高（17.92 至 29.16mg/g），绿原酸是最丰富的酚类化合物（10.48 至 13.99mg/g）。丙酮提取物的总酚、类黄酮和皂苷含量最高，抗氧化活性最强。此外，所有提取物均对 HepG2 细胞内 ROS 生成的抑制具有良好的作用。根据 DPPH•-UHPLC-HRMS 和 ABTS•+-UHPLC-HRMS 分析的结果，酚类化合物而非皂苷对三叶崖爬藤茎的 DPPH 或 ABTS 自由基清除活性有显著贡献。这些结果可能有助于进一步理解和利用三叶崖爬藤茎作为食品或营养保健品行业的潜在天然来源。
