Laboratory of Flavor and Chromatographic Analysis, PROCTA, Federal University of Sergipe, 49100-000 São Cristóvão, SE, Brazil.
Laboratory of Flavor and Chromatographic Analysis, PROCTA, Federal University of Sergipe, 49100-000 São Cristóvão, SE, Brazil.
Food Res Int. 2021 May;143:110282. doi: 10.1016/j.foodres.2021.110282. Epub 2021 Mar 9.
The aim of this study was to analyze eight selected species of leaves, used in the traditional medicine of the Northeast region of Brazil obtained from several fruit trees (grageru, soursop, jambolanum, passion fruit, insulin, nogueira, pedra ume kaá and stévia), regarding their polyphenols contents and antioxidant activity. Condensed and hydrolysable tannins, phenolics and flavonoids contents were determined and the antioxidant activities measured by ABTS, FRAP and ORAC assays. Organic acids were analyzed by HPLC-DAD system. Phenolic compounds of aqueous and ethanolic extracts were determined by UHPLC-DAD-MS. The results revealed high contents of total phenolics (13.34 ± 0.19 - 127.65 ± 0.21 mg.g of GAE:QE (2:1) of sample) and flavonoids (12.30 ± 0.42 - 71.79 ± 0.00 mg.g QE of sample). The ABTS results exhibited extraordinary activity in the extracts (74.48 ± 6.23 - 1487.33 ± 2.67 µmol Trolox.g of sample). Acids quinic, tartaric, citric, gallic, chlorogenic, p-coumaric, ferulic and vanillic along with naringenin, rutin, vanillin, catechin, epicatechin, kaempferol were the most important compounds. Thus, these leaves extracts may be considered as sources of phenolics compounds having a high potential as natural antioxidants. In addition, the polyphenols present in these leaves have many beneficial effects and can also be used in medicinal and nutraceuticals products with enhanced bioactivities.
本研究旨在分析八种选自巴西东北部传统医学的叶片，这些叶片来自多种果树（grageru、番荔枝、鸦胆子、西番莲、胰岛素、巴西坚果、梅卡乌姆和甜叶菊），检测其多酚含量和抗氧化活性。测定了缩合单宁和可水解单宁、酚类和类黄酮的含量，并通过 ABTS、FRAP 和 ORAC 测定法测定了抗氧化活性。采用 HPLC-DAD 系统分析有机酸。通过 UHPLC-DAD-MS 测定水提物和醇提物中酚类化合物的含量。结果表明，总酚（13.34±0.19-127.65±0.21 mgGAE：QE（2:1））和类黄酮（12.30±0.42-71.79±0.00 mgQE）含量均较高。ABTS 结果显示提取物具有非凡的活性（74.48±6.23-1487.33±2.67 µmol Trolox.g）。奎宁酸、酒石酸、柠檬酸、没食子酸、绿原酸、对香豆酸、阿魏酸和香草酸以及柚皮苷、芦丁、香草醛、儿茶素、表儿茶素、山奈酚是最重要的化合物。因此，这些叶片提取物可作为具有高抗氧化潜力的多酚化合物的来源。此外，这些叶片中的多酚具有许多有益的作用，也可用于增强生物活性的药用和营养保健品中。
