多糖结构和构象的 NMR 分析综述：进展、挑战与展望。
A review of NMR analysis in polysaccharide structure and conformation: Progress, challenge and perspective.
机构信息
State Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology (Nanchang), Nanchang University, 235 Nanjing East Road, Nanchang 330047, China.
State Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology (Nanchang), Nanchang University, 235 Nanjing East Road, Nanchang 330047, China; National R&D Center for Freshwater Fish Processing, Jiangxi Normal University, Nanchang, Jiangxi 330022, China.
出版信息
Food Res Int. 2021 May;143:110290. doi: 10.1016/j.foodres.2021.110290. Epub 2021 Mar 9.
Nuclear magnetic resonance (NMR) has been widely used as an analytical chemistry technique to investigate the molecular structure and conformation of polysaccharides. Combined with 1D spectra, chemical shifts and coupling constants in both homo- and heteronuclear 2D NMR spectra are able to infer the linkage and sequence of sugar residues. Besides, NMR has also been applied in conformation, quantitative analysis, cell wall in situ, degradation, polysaccharide mixture interaction analysis, as well as carbohydrates impurities profiling. This review summarizes the principle and development of NMR in polysaccharides analysis, and provides NMR spectra data collections of some common polysaccharides. It will help to promote the application of NMR in complex polysaccharides of biochemical interest, and provide valuable information on commercial polysaccharide products.
核磁共振（NMR）已被广泛用作分析化学技术，用于研究多糖的分子结构和构象。结合一维谱，同核和异核二维 NMR 谱中的化学位移和偶合常数能够推断糖残基的连接和顺序。此外，NMR 还应用于构象、定量分析、细胞壁原位、降解、多糖混合物相互作用分析以及碳水化合物杂质分析。本文综述了 NMR 在多糖分析中的原理和发展，并提供了一些常见多糖的 NMR 谱数据集。这将有助于推动 NMR 在生化感兴趣的复杂多糖中的应用，并为商业多糖产品提供有价值的信息。
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