IRCM, Institut de Recherche en Cancérologie de Montpellier, INSERM U1194, Université Montpellier, Montpellier F-34298, France.
LBN, Univ Montpellier, Montpellier, France.
Food Chem. 2021 Oct 30;360:130003. doi: 10.1016/j.foodchem.2021.130003. Epub 2021 May 5.
TiO nanoparticles known as E171 are one controversial food additive due to its potential toxicity. In this work, the main hypothesis is that the proteins adsorbed on the TiO nanoparticles prevent their aggregation and favor the cell penetration. To do so, the TiO nanoparticles were coated with gelatin and β-lactoglobulin to reach interfacial concentrations about 0.25 mg/mg and 0.32 mg/mg, respectively. The measurement of NP size showed that the protein coating improve the colloidal stability of TiO nanoparticles. The FTIR analysis suggests that the β-lactoglobulin structure is modified after adsorption. The penetration of TiO penetration inside human intestinal epithelial cells was shown and quantify by using confocal Raman microscopy. The promoting role of the protein coating on the cell penetration was demonstrated for both the gelatin and β-lactoglobulin. Finally, the results allow establishing a correlation between the ability of proteins to prevent NP aggregation and the cell penetration.
TiO 纳米颗粒，俗称 E171，因其潜在毒性而成为一种备受争议的食品添加剂。在这项工作中，主要假设是吸附在 TiO 纳米颗粒上的蛋白质阻止了它们的聚集，并有利于细胞穿透。为此，将 TiO 纳米颗粒用明胶和β-乳球蛋白进行包覆，达到界面浓度分别约为 0.25mg/mg 和 0.32mg/mg。纳米颗粒尺寸的测量表明，蛋白质包覆改善了 TiO 纳米颗粒的胶体稳定性。FTIR 分析表明，β-乳球蛋白的结构在吸附后发生了修饰。利用共聚焦拉曼显微镜显示和定量研究了 TiO 纳米颗粒穿透入人体肠上皮细胞的情况。结果表明，明胶和β-乳球蛋白的蛋白质包覆都对细胞穿透起到了促进作用。最后，结果表明，蛋白质防止 NP 聚集的能力与细胞穿透之间存在相关性。
