Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8572, Japan.
Department of Biology, National Museum of Nature and Science, 4-4-1 Amakubo, Tsukuba, Ibaraki, 305-0005, Japan.
J Nat Med. 2021 Sep;75(4):877-883. doi: 10.1007/s11418-021-01531-z. Epub 2021 May 16.
Two new compounds, podogigants A (1) and B (2), were isolated from the culture broth of Podostroma giganteum. This is the first report on the identification of secondary metabolites in P. giganteum. The structures of 1 and 2 were elucidated through spectroscopic analysis, including 2D NMR spectroscopy assisted by chemical derivatization, which revealed the presence of farnesyl- and geranyl-hydroquinone structures, respectively. Compounds 1 and 2 exhibited no antifungal activity even at a concentration of 64 μg/mL, whereas they potentiated amphotericin B (AmB) activity against several species of fungi. In particular, 1 potentiated AmB activity against C. albicans and R. oryzae by up to 32-fold (MIC value of AmB decreased from 1.0 to 0.032 µg/mL), while 2 potentiated AmB activity against C. albicans by up to 16-fold.
从大秃马勃（Podostroma giganteum）的发酵液中分离得到了两种新的化合物，分别命名为 podogigants A（1）和 B（2）。这是首次报道大秃马勃中次级代谢产物的鉴定。通过光谱分析，包括化学衍生化辅助的 2D NMR 光谱学，阐明了 1 和 2 的结构，分别揭示了法呢基和香叶基对苯二酚结构的存在。化合物 1 和 2 即使在 64μg/mL 的浓度下也没有显示出抗真菌活性，而它们对几种真菌的两性霉素 B（AmB）活性具有增效作用。特别是，1 使 AmB 对白色念珠菌和稻瘟病菌的活性增强了 32 倍（AmB 的 MIC 值从 1.0 降至 0.032μg/mL），而 2 使 AmB 对白色念珠菌的活性增强了 16 倍。
