College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China.
College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China.
Pestic Biochem Physiol. 2021 Jun;175:104851. doi: 10.1016/j.pestbp.2021.104851. Epub 2021 Apr 7.
The insect voltage-gated sodium channel is the primary target of pyrethroids and novel efficient insecticides such as indoxacarb and metaflumizone. In this study, we cloned and characterized two putative sodium channel genes, TcNav1 and TcNav2, in Tribolium castaneum. The composite TcNav1 and TcNav2 encode a protein of 2045 and 2037 amino acid residues, sharing 76.1% and 75.5% amino acid identity with Drosophila para, respectively. Comparative analysis of genomic organization showed that TcNav1 and TcNav2 contain 26 and 27 exons, respectively. Analysis of the expression patterns showed that the mRNA levels of TcNav1 and TcNav2 were predominantly expressed in the head. RNAi-mediated knockdown of both TcNav1 and TcNav2 adversely affected adult emergence and significantly decreased sensitivity to deltamethrin. Significantly reduced pupation rate and sensitivity to beta-cypermethrin were observed after injection of siRNA targeting TcNav1 but not TcNav2. Taken together, we provide evidence that sodium channel gene has undergone duplication in T. castaneum, resulting in diversified developmental and toxicological functions.
昆虫电压门控钠离子通道是拟除虫菊酯和新型高效杀虫剂如茚虫威和甲氰菊酯的主要靶标。在这项研究中，我们在赤拟谷盗中克隆和表征了两个假定的钠离子通道基因 TcNav1 和 TcNav2。复合 TcNav1 和 TcNav2 编码一个由 2045 和 2037 个氨基酸残基组成的蛋白质，分别与果蝇 para 具有 76.1%和 75.5%的氨基酸同一性。基因组组织的比较分析表明，TcNav1 和 TcNav2 分别包含 26 和 27 个外显子。表达模式分析表明，TcNav1 和 TcNav2 的 mRNA 水平主要在头部表达。RNAi 介导的 TcNav1 和 TcNav2 敲低均对成虫的出现产生不利影响，并显著降低了对溴氰菊酯的敏感性。注射靶向 TcNav1 的 siRNA 后，显著降低了蛹化率和对β-氯氰菊酯的敏感性，但对 TcNav2 没有影响。综上所述，我们提供了证据表明，钠离子通道基因在赤拟谷盗中发生了复制，导致了多样化的发育和毒理学功能。
