Research Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China.
Research Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China; School of Life Science, Shanxi University, Taiyuan, Shanxi 030006, China.
Pestic Biochem Physiol. 2021 Jun;175:104854. doi: 10.1016/j.pestbp.2021.104854. Epub 2021 Apr 12.
Chitin deacetylases including CDA1 and CDA2, containing a chitin deacetylase domain and an LDL domain, have been reported to be essential for cuticle structure differentiation in different insect species. However, it is yet unexplored whether CDA1 and CDA2 activity is needed for the function of the cuticle as a barrier against pathogen and xenobiotics penetration. In this study, we studied the efficiency of fungal infection in the migratory locust Locusta migratoria in dependence of LmCDA1 and LmCDA2 function. Second instar nymphs injected with dsRNA against LmCDA1 and LmCDA2 transcripts were less resistant against the infection by the fungus Metarhizium anisopliae than control nymphs. At the same time, permeability to organophosphorus pesticides was increased in these nymphs. Interestingly, the CHC amounts at the cuticle surface were unaffected upon LmCDA1 and LmCDA2 reduction. These results suggest that the barrier function of the locust cuticle not only depends on surface CHCs, but also on an intact procuticle.
壳质脱乙酰酶包括 CDA1 和 CDA2，它们含有壳质脱乙酰酶结构域和 LDL 结构域，已被报道对不同昆虫物种的表皮结构分化至关重要。然而，CDA1 和 CDA2 活性是否对表皮作为阻止病原体和异生物穿透的屏障的功能是必需的，这尚未得到探索。在这项研究中，我们研究了 LmCDA1 和 LmCDA2 功能对迁徙蝗 Locusta migratoria 真菌感染效率的影响。用针对 LmCDA1 和 LmCDA2 转录本的 dsRNA 注射的 2 龄若虫对真菌绿僵菌的感染的抵抗力比对照若虫低。同时，这些若虫对有机磷农药的渗透性增加。有趣的是，在 LmCDA1 和 LmCDA2 减少的情况下，表皮表面的 CHC 数量不受影响。这些结果表明，蝗虫表皮的屏障功能不仅依赖于表面 CHC，还依赖于完整的原表皮。
