Center for Cell Biology and Tissue Engineering, Institute for Chemistry and Biotechnology, Zurich University of Applied Sciences, Wädenswil, Switzerland.
Regenerative, Modular, and Developmental Engineering Laboratory (REMODEL), National University of Ireland Galway (NUI Galway), Galway, Ireland; Science Foundation Ireland (SFI) Centre for Research in Medical Devices (CÚRAM), National University of Ireland Galway (NUI Galway), Galway, Ireland; Regenerative, Modular, and Developmental Engineering Laboratory (REMODEL), Faculty of Biomedical Sciences, Università della Svizzera Italiana (USI), Lugano, Switzerland; Regenerative, Modular, and Developmental Engineering Laboratory (REMODEL), School of Mechanical and Materials Engineering, University College Dublin (UCD), Dublin, Ireland.
Trends Biochem Sci. 2021 Oct;46(10):805-811. doi: 10.1016/j.tibs.2021.04.006. Epub 2021 May 13.
In multicellular organisms, the intracellular and extracellular spaces are considerably packed with a diverse range of macromolecular species. Yet, standard eukaryotic cell culture is performed in dilute, and deprived of macromolecules culture media, that barely imitate the density and complex macromolecular composition of tissues. Essentially, we drown cells in a sea of media and then expect them to perform physiologically. Herein, we argue the use of macromolecular crowding (MMC) in eukaryotic cell culture for regenerative medicine and drug discovery purposes.
在多细胞生物中，细胞内和细胞外空间都被大量的各种大分子物质所填充。然而，标准的真核细胞培养是在稀释的、缺乏大分子物质的培养基中进行的，这些培养基几乎无法模拟组织的密度和复杂的大分子组成。从本质上讲，我们将细胞淹没在培养基的海洋中，然后期望它们发挥生理功能。在此，我们主张在真核细胞培养中使用大分子拥挤（MMC）来进行再生医学和药物发现的研究。
