Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine, Hyogo, Japan.
TechnoSuruga Laboratory Co., Ltd, Shizuoka, Japan.
Kobe J Med Sci. 2021 Jan 4;66(4):E139-E148.
Daikenchuto (TU-100) is herbal medicine which predominantly contains ginger, Japanese pepper, and ginseng. We investigated whether TU-100 can affect the composition of gut flora and intestinal tumor development using Apc mice, a murine model of intestinal tumor. Bacterial 16S rRNA sequencing and short-chain fatty acid analysis were performed on faecal samples. Tumor number and size were analysed. Any change in gene expression of the tumor tissues was assessed by real-time PCR. Principal coordinate analysis (PCoA) showed that the faecal microbiota cluster of TU-100-fed mice was different from the microbiota of control mice. However, no significant difference was observed in the concentration of short-chain fatty acids, tumor number, and gene expression levels between the two groups. Our data showed that TU-100 can affect the intestinal environment; however, it does not contribute in tumor progression or inhibition in our setting.
大建中汤（TU-100）是一种主要含有生姜、日本胡椒和人参的草药。我们使用 Apc 小鼠（一种肠道肿瘤的小鼠模型）研究了 TU-100 是否会影响肠道菌群的组成和肠道肿瘤的发展。对粪便样本进行了细菌 16S rRNA 测序和短链脂肪酸分析。分析了肿瘤数量和大小。通过实时 PCR 评估了肿瘤组织中基因表达的任何变化。主坐标分析（PCoA）显示，接受 TU-100 喂养的小鼠的粪便微生物群与对照组的微生物群不同。然而，两组间短链脂肪酸浓度、肿瘤数量和基因表达水平没有显著差异。我们的数据表明，TU-100 可以影响肠道环境；然而，在我们的研究中，它并没有促进或抑制肿瘤的进展。
