Jay D G
Department of Neurobiology, Harvard Medical School, Boston, MA 02115.
Proc Natl Acad Sci U S A. 1988 Aug;85(15):5454-8. doi: 10.1073/pnas.85.15.5454.
Chromophore-assisted laser inactivation of protein function has been achieved. After a protein binds a specific ligand or antibody conjugated with malachite green (C.I. 42,000), it is selectively inactivated by laser irradiation at a wavelength of light absorbed by the dye but not significantly absorbed by cellular components. Ligand-bound proteins in solution and on the surfaces of cells can be denatured without other proteins in the same samples being affected. Chromophore-assisted laser inactivation can be used to study cell surface phenomena by inactivating the functions of single proteins on living cells, a molecular extension of cellular laser ablation. It has an advantage over genetics and the use of specific inhibitors in that the protein function of a single cell within the organism can be inactivated by focusing the laser beam.
已经实现了发色团辅助的蛋白质功能激光失活。蛋白质与与孔雀石绿（C.I. 42,000）偶联的特定配体或抗体结合后，通过在染料吸收的波长处进行激光照射而被选择性失活，而细胞成分对该波长的光吸收不明显。溶液中和细胞表面上与配体结合的蛋白质可以变性，而同一样品中的其他蛋白质不受影响。发色团辅助的激光失活可用于通过使活细胞上单个蛋白质的功能失活来研究细胞表面现象，这是细胞激光烧蚀的分子扩展。与遗传学和使用特定抑制剂相比，它具有一个优势，即通过聚焦激光束可以使生物体中单个细胞的蛋白质功能失活。
