Brosius J, Arfsten U
Biochemistry. 1978 Feb 7;17(3):508-16. doi: 10.1021/bi00596a021.
Protein L19, a component of the Escherichia coli 50S ribosomal subunit implicated in 30S-50S subunit interaction was sequenced by the dansyl-Edman method. L19 consists of a single polypeptide chain of 114 amino acids giving a calculated molecular weight of 13 002. Peptides obtained from various enzymatic cleavages were isolated on thin-layer peptide maps or gel filtration. Automated Edman degradation using a liquid phase sequenator was carried out on the whole protein as well as on a large 58-residue fragment arising from digestion with Staphylococcus aureus protease. Every position in protein L19 was confirmed at least twice. Results of secondary structure estimation and homologies with other E. coli ribosomal protein sequences are presented.
蛋白质L19是大肠杆菌50S核糖体亚基的一个组成部分，与30S - 50S亚基相互作用有关，采用丹磺酰 - 埃德曼法对其进行了测序。L19由一条含114个氨基酸的单多肽链组成，计算分子量为13002。通过各种酶切获得的肽段在薄层层析肽图或凝胶过滤上进行分离。使用液相测序仪对整个蛋白质以及由金黄色葡萄球菌蛋白酶消化产生的一个58个残基的大片段进行自动埃德曼降解。蛋白质L19中的每个位置至少被确认了两次。给出了二级结构估计结果以及与其他大肠杆菌核糖体蛋白序列的同源性。
