Brosius J
Biochemistry. 1978 Feb 7;17(3):501-8. doi: 10.1021/bi00596a020.
Protein L31 from the 50S ribosomal subunit of Escherichia coli was manually sequenced by the dansyl-Edman method. Owing to the availability of only small quantities of purified L31, sequencing methods were scaled down such that the entire primary structure could be determined with 700 microgram of protein. The techniques employed are described in detail. The protein consists of a single chain of 62 amino acids, with a calculated molecular weight of 6967. Four half-cystine residues were identified at positions 16, 18, 37, and 40. Evidence is presented that suggests that these residues form two disulfide bridges in the protein, as isolated.
通过丹磺酰-埃德曼法对大肠杆菌50S核糖体亚基中的L31蛋白进行了手工测序。由于仅能获得少量纯化的L31，测序方法按比例缩小，以便能用700微克蛋白质确定其完整的一级结构。文中详细描述了所采用的技术。该蛋白由一条62个氨基酸的单链组成，计算分子量为6967。在第16、18、37和40位鉴定出四个半胱氨酸残基。有证据表明，这些残基在分离出的蛋白中形成了两个二硫键。
