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黄瓜核糖体蛋白CsRPS21与葫芦褪绿黄化病毒的P22蛋白相互作用。

Cucumber Ribosomal Protein CsRPS21 Interacts With P22 Protein of Cucurbit Chlorotic Yellows Virus.

作者信息

Yang Xue, Wei Ying, Shi Yajuan, Han Xiaoyu, Chen Siyu, Yang Lingling, Li Honglian, Sun Bingjian, Shi Yan

机构信息

College of Plant Protection, Henan Agricultural University, Zhengzhou, China.

出版信息

Front Microbiol. 2021 Apr 29;12:654697. doi: 10.3389/fmicb.2021.654697. eCollection 2021.

DOI:10.3389/fmicb.2021.654697
PMID:33995313
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8116660/
Abstract

Cucurbit chlorotic yellows virus (CCYV) is a cucurbit-infecting crinivirus. RNA silencing can be initiated as a plant defense against viruses. Viruses encode various RNA silencing suppressors to counteract antiviral silencing. P22 protein encoded by RNA1 of CCYV is a silencing suppressor, but its mechanism of action remains unclear. In this study, the cucumber ribosomal-like protein CsRPS21 was found to interact with P22 protein and . A conserved CsRPS21 domain was indispensable for its nuclear localization and interaction with P22. Transient expression of CsRPS21 in leaves interfered with P22 accumulation and inhibited P22 silencing suppressor activity. CsRPS21 expression in protoplasts inhibited CCYV accumulation. Increasing numbers of ribosomal proteins are being found to be involved in viral infections of plants. We identified a P22-interacting ribosomal protein, CsRPS21, and uncovered its role in early viral replication and silencing suppressor activity. Our study increases knowledge of the function of ribosomal proteins during viral infection.

摘要

南瓜褪绿黄化病毒（CCYV）是一种感染葫芦科作物的毛形病毒。RNA沉默可作为植物抵御病毒的一种防御机制启动。病毒编码多种RNA沉默抑制子以对抗抗病毒沉默。CCYV的RNA1编码的P22蛋白是一种沉默抑制子，但其作用机制尚不清楚。在本研究中，发现黄瓜核糖体样蛋白CsRPS21与P22蛋白相互作用。保守的CsRPS21结构域对于其核定位及与P22的相互作用必不可少。CsRPS21在叶片中的瞬时表达干扰了P22的积累并抑制了P22沉默抑制子的活性。CsRPS21在原生质体中的表达抑制了CCYV的积累。越来越多的核糖体蛋白被发现参与植物的病毒感染。我们鉴定出一种与P22相互作用的核糖体蛋白CsRPS21，并揭示了其在病毒早期复制和沉默抑制子活性中的作用。我们的研究增加了对核糖体蛋白在病毒感染过程中功能的认识。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e6b/8116660/b00900e43d6a/fmicb-12-654697-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e6b/8116660/406302410833/fmicb-12-654697-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e6b/8116660/d4085841cd9c/fmicb-12-654697-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e6b/8116660/a0994f9e59d3/fmicb-12-654697-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e6b/8116660/c82edfd58914/fmicb-12-654697-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e6b/8116660/b00900e43d6a/fmicb-12-654697-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e6b/8116660/406302410833/fmicb-12-654697-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e6b/8116660/d4085841cd9c/fmicb-12-654697-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e6b/8116660/a0994f9e59d3/fmicb-12-654697-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e6b/8116660/c82edfd58914/fmicb-12-654697-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e6b/8116660/b00900e43d6a/fmicb-12-654697-g005.jpg

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2
Plastid ribosomal protein LPE2 is involved in photosynthesis and the response to C/N balance in Arabidopsis thaliana.质体核糖体蛋白 LPE2 参与拟南芥的光合作用和 C/N 平衡响应。
J Integr Plant Biol. 2020 Sep;62(9):1418-1432. doi: 10.1111/jipb.12907. Epub 2020 Mar 17.
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Cucurbit chlorotic yellows virus p22 is a suppressor of local RNA silencing.
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Mol Plant Pathol. 2023 Sep;24(9):1047-1062. doi: 10.1111/mpp.13347. Epub 2023 May 6.
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