Landeo-Ríos Yazmín, Navas-Castillo Jesús, Moriones Enrique, Cañizares M Carmen
Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora"-Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Estación Experimental "La Mayora", 29750 Algarrobo-Costa, Málaga, Spain.
Viruses. 2016 Jun 28;8(7):182. doi: 10.3390/v8070182.
Among the components of the RNA silencing pathway in plants, RNA-dependent RNA polymerases (RDRs) play fundamental roles in antiviral defence. Here, we demonstrate that the Nicotiana benthamiana RDR6 is involved in defence against the bipartite crinivirus (genus Crinivirus, family Closteroviridae) Tomato chlorosis virus (ToCV). Additionally, by producing a p22-deficient ToCV infectious mutant clone (ToCVΔp22), we studied the role of this viral suppressor of RNA silencing in viral infection in both wild-type and RDR6-silenced N. benthamiana (NbRDR6i) plants. We demonstrate that p22 is dispensable for the replication of ToCV, where RDR6 appears not to have any effect. Furthermore, the finding that ToCV∆p22 systemic accumulation was impaired in wild-type N. benthamiana but not in NbRDR6i plants suggests a role for p22 in counteracting an RDR6-mediated antiviral response of the plant during systemic infection.
在植物RNA沉默途径的组分中，RNA依赖性RNA聚合酶（RDR）在抗病毒防御中发挥着重要作用。在此，我们证明本氏烟草RDR6参与了对二分体纤细病毒（纤细病毒属，长线形病毒科）番茄褪绿病毒（ToCV）的防御。此外，通过构建一个缺乏p22的ToCV感染性突变体克隆（ToCVΔp22），我们研究了这种RNA沉默病毒抑制因子在野生型和RDR6沉默的本氏烟草（NbRDR6i）植株病毒感染中的作用。我们证明p22对于ToCV的复制并非必需，而RDR6似乎对此没有任何影响。此外，ToCV∆p22在野生型本氏烟草中系统积累受损，但在NbRDR6i植株中未受损，这一发现表明p22在系统感染期间对抗植物RDR6介导的抗病毒反应中发挥作用。
