Qadri Zeba, Righi Valeria, Li Shasha, Tzika A Aria
MGH NMR Surgical Laboratory, Center for Surgery, Innovation and Bioengineering, Department of Surgery, Massachusetts General and Shriners Burn Hospitals, Harvard Medical School, Boston, USA.
Athinoula A. Martinos Center of Biomedical Imaging, Department of Radiology, Massachusetts General Hospital, Harvard Medical School Charlestown, USA.
Adv J Mol Imaging. 2021 Jan;11(1):1-15. doi: 10.4236/ami.2021.111001.
Therapies based on stem cell transplants offer significant potential in the field of regenerative medicine. Monitoring the fate of the transplanted stem cells in a timely manner is considered one of the main limitations for long-standing success of stem cell transplants. Imaging methods that visualize and track stem cells non-invasively in real time are helpful towards the development of successful cell transplantation techniques. Novel molecular imaging methods which are non-invasive particularly such as MRI have been of great recent interest. Hence, mouse models which are of clinical relevance have been studied by injecting contrast agents used for labelling cells such as super-paramagnetic iron-oxide (SPIO) nanoparticles for cellular imaging. The MR techniques which can be used to generate positive contrast images have been of much relevance recently for tracking of the labelled cells. Particularly when the off-resonance region in the vicinity of the labeled cells is selectively excited while suppressing the signals from the non-labeled regions by the method of spectral dephasing. Thus, tracking of magnetically labelled cells employing positive contrast MR imaging methods in a burn mouse model in a non-invasive way has been the scope of this study. The consequences have direct implications for monitoring labeled stem cells at some stage in wound healing. We suggest that our approach can be used in clinical trials in molecular and regenerative medicine.
基于干细胞移植的疗法在再生医学领域具有巨大潜力。及时监测移植干细胞的命运被认为是干细胞移植长期成功的主要限制之一。能够实时非侵入性地可视化和追踪干细胞的成像方法有助于成功细胞移植技术的发展。特别是非侵入性的新型分子成像方法，如磁共振成像（MRI），最近备受关注。因此，通过注射用于标记细胞的造影剂，如用于细胞成像的超顺磁性氧化铁（SPIO）纳米颗粒，对具有临床相关性的小鼠模型进行了研究。最近，可用于生成阳性对比图像的磁共振技术对于追踪标记细胞具有重要意义。特别是当通过频谱去相位方法选择性激发标记细胞附近的失谐区域，同时抑制来自未标记区域的信号时。因此，本研究的范围是以非侵入性方式在烧伤小鼠模型中采用阳性对比磁共振成像方法追踪磁性标记细胞。这些结果对在伤口愈合的某个阶段监测标记干细胞具有直接影响。我们认为我们的方法可用于分子和再生医学的临床试验。
