在体内观察干细胞的工作情况。
Seeing stem cells at work in vivo.
机构信息
Russell H. Morgan Department of Radiology and Radiological Science, Division of MR Research, The Johns Hopkins University School of Medicine, 217 Traylor Building, 720 Rutland Avenue, Baltimore, MD, 21205-1832, USA.
出版信息
Stem Cell Rev Rep. 2014 Feb;10(1):127-44. doi: 10.1007/s12015-013-9468-x.
Abstract
Stem cell based-therapies are novel therapeutic strategies that hold key for developing new treatments for diseases conditions with very few or no cures. Although there has been an increase in the number of clinical trials involving stem cell-based therapies in the last few years, the long-term risks and benefits of these therapies are still unknown. Detailed in vivo studies are needed to monitor the fate of transplanted cells, including their distribution, differentiation, and longevity over time. Advancements in non-invasive cellular imaging techniques to track engrafted cells in real-time present a powerful tool for determining the efficacy of stem cell-based therapies. In this review, we describe the latest approaches to stem cell labeling and tracking using different imaging modalities.
摘要
基于干细胞的疗法是一种新颖的治疗策略，为开发治疗方法很少或没有治愈方法的疾病提供了关键。尽管在过去几年中，涉及基于干细胞的疗法的临床试验数量有所增加，但这些疗法的长期风险和益处仍不清楚。需要进行详细的体内研究来监测移植细胞的命运，包括它们的分布、分化和随时间的持久性。实时跟踪移植细胞的非侵入性细胞成像技术的进步为确定基于干细胞的疗法的疗效提供了强大的工具。在这篇综述中，我们描述了使用不同成像方式对干细胞进行标记和跟踪的最新方法。
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