Nichols Zach E, Saha Lahari, Knoblauch Rachael, Santaus Tonya M, Geddes Chris D
Department of Chemistry and Biochemistry, University of Maryland, Baltimore County, Catonsville, MD 21250, USA.
Institute of Fluorescence, Baltimore, MD 21202, USA.
IEEE Access. 2021;9:37823-37833. doi: 10.1109/access.2021.3063092. Epub 2021 Mar 8.
Sample preparation is one of the most time-consuming steps in diagnostic assays, particularly those involving biological samples. In this paper we report the results of finite-difference time-domain (FDTD) simulations and thermographic imaging experiments carried out with the intent of designing a microplate for rapid, high-throughput sample preparation to aid diagnostic assays. This work is based on devices known as microwave lysing triangles (MLTs) that have been proven capable of rapid sample preparation when irradiated in a standard microwave cavity. FDTD software was used to model a microplate platform as a polystyrene substrate with an array of various passive scattering elements (PSEs) of different sizes, shapes, and interelement spacings in a 2.45 GHz field identical to that of a common microwave oven. Based on the FDTD modeling, several PSE arrays were fabricated by cutting PSEs out of aluminum foil and adhering them to the bottom of regular polystyrene microplates to make prototypes. Each prototype microplate was then irradiated in a microwave cavity for a range of different times, powers, and source angles and the heating effects were observed via a forward looking infrared (FLIR) camera. Based on the results, two prototype microplate platforms have been shown to demonstrate electromagnetic and thermal enhancements similar to those seen with MLTs as well as tunable thermal responses to radio frequency (RF) irradiation.
样品制备是诊断检测中最耗时的步骤之一，尤其是那些涉及生物样品的检测。在本文中，我们报告了时域有限差分（FDTD）模拟和热成像实验的结果，这些实验旨在设计一种用于快速、高通量样品制备以辅助诊断检测的微孔板。这项工作基于一种称为微波裂解三角（MLT）的装置，该装置已被证明在标准微波腔中辐照时能够快速进行样品制备。FDTD软件用于将微孔板平台建模为聚苯乙烯基板，在与普通微波炉相同的2.45 GHz场中，该基板带有一系列不同尺寸、形状和元件间距的各种无源散射元件（PSE）阵列。基于FDTD建模，通过从铝箔上切割出PSE并将它们粘贴到普通聚苯乙烯微孔板底部来制造几个PSE阵列，以制作原型。然后，每个原型微孔板在微波腔中以一系列不同的时间、功率和源角度进行辐照，并通过前视红外（FLIR）相机观察加热效果。基于这些结果，已证明两个原型微孔板平台表现出与MLT类似的电磁和热增强效果，以及对射频（RF）辐照的可调热响应。
