Liang Chaoan, Jiang Qiming, Yu Yi, Xu Tao, Sun Hanyu, Deng Feilong, Yu Xiaolin
Guangdong Provincial Key Laboratory of Stomatology, Department of Oral Implantology, Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, China.
Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Key Laboratory for Advanced Materials Processing Technology, Ministry of Education, Department of Mechanical Engineering, Tsinghua University, Beijing, China.
Front Bioeng Biotechnol. 2021 Apr 29;9:676874. doi: 10.3389/fbioe.2021.676874. eCollection 2021.
Lithium (Li) reportedly has anti-bacterial properties. Thus, it is an ideal option to modify barrier membranes used for guided bone regeneration to inhibit the bacterial adhesion. The aims of this study were to fabricate and characterize nanofibrous poly(L-lactic acid) (PLLA) membranes containing Li, and investigate their antibacterial effects on and . Li (5%Li, 10%Li, and 15%Li)-loaded nanofibrous PLLA membranes were fabricated using an electrospinning technique, and characterized via scanning electron microscopy, X-ray photoelectron spectroscopy, X-ray diffraction, a contact angle measuring device, and a universal testing machine. Sustained release of Li ions was measured over a 14-day period and biocompatibility of the Li-PLLA membranes was investigated. Evaluation of bacterial adhesion and antibacterial activity were conducted by bacterial colony counting, LIVE/DEAD staining and inhibition zone method using and . Of the three Li-loaded membranes assessed, the 10%Li-PLLA membrane had the best mechanical properties and biocompatibility. Adhesion of both and on Li-PLLA membranes was significantly lower than adhesion on pure PLLA membranes, particularly with regard to the 10%Li and 15%Li membranes. Significant antibacterial activity of Li-PLLA were also observed against according to the inhibition zone test. Given their better mechanical properties, biocompatibility, and antibacterial activity, PLLAs with 10%Li are a better choice for future clinical utilization. The pronounced antibacterial effects of Li-loaded PLLA membranes sets the stage for further application in guided bone regeneration.
据报道，锂（Li）具有抗菌特性。因此，它是修饰用于引导骨再生的屏障膜以抑制细菌粘附的理想选择。本研究的目的是制备并表征含锂的纳米纤维聚（L-乳酸）（PLLA）膜，并研究它们对[具体细菌名称1]和[具体细菌名称2]的抗菌效果。采用静电纺丝技术制备了负载锂（5%Li、10%Li和15%Li）的纳米纤维PLLA膜，并通过扫描电子显微镜、X射线光电子能谱、X射线衍射、接触角测量仪和万能试验机对其进行表征。在14天的时间内测量了锂离子的持续释放情况，并研究了锂-PLLA膜的生物相容性。通过菌落计数、活/死染色和抑菌圈法，使用[具体细菌名称1]和[具体细菌名称2]对细菌粘附和抗菌活性进行了评估。在评估的三种负载锂的膜中，10%Li-PLLA膜具有最佳的机械性能和生物相容性。[具体细菌名称1]和[具体细菌名称2]在锂-PLLA膜上的粘附力明显低于在纯PLLA膜上的粘附力，特别是对于10%Li和15%Li的膜。根据抑菌圈试验，还观察到锂-PLLA对[具体细菌名称1]具有显著的抗菌活性。鉴于其更好的机械性能、生物相容性和抗菌活性，含10%Li的PLLA是未来临床应用的更好选择。负载锂的PLLA膜的显著抗菌效果为其在引导骨再生中的进一步应用奠定了基础。
