Li Bo, Chen Ying, He Jisu, Zhang Jing, Wang Song, Xiao Wenqian, Liu Zhongning, Liao Xiaoling
Chongqing Key Laboratory of Nano/Micro Composite Materials and Devices, Chongqing Engineering Laboratory of Nano/Micro Biomedical Detection Technology, Chongqing University of Science and Technology, Chongqing 401331, China.
Department of Prosthodontics, National Clinical Research Center for Oral Diseases, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Peking University School and Hospital of Stomatology, Beijing 100081, China.
ACS Biomater Sci Eng. 2020 Dec 14;6(12):6737-6747. doi: 10.1021/acsbiomaterials.0c00972. Epub 2020 Nov 9.
Nanofibrous poly(l-lactic acid) (PLLA) membrane-simulated extracellular matrices (ECMs) can be used in the biomedical field. However, the hydrophobic nature and poor osteoinductive property of PLLA limit its application in guided bone regeneration (GBR). In this work, a methacrylated gelatin/nano-HA (GelMA/nHA) complex was first synthesized in situ and then introduced into PLLA to fabricate biomimetic GelMA/nHA/PLLA membranes, mimicking the nanofibrous architecture and composition of ECMs by electrospinning and photocrosslinking. Compared to PLLA and GelMA/PLLA membranes, the novel GelMA/nHA/PLLA membranes demonstrated better tensile, hydrophilic, water sorption, and degradation properties. An in vitro biological evaluation indicated that the membranes promoted human bone marrow-derived mesenchymal stem cell (hBMSC) proliferation, adhesion, and osteogenic differentiation. Critical-sized defects in rat models were used to evaluate the bone regeneration performances of the three kinds of membranes in vivo, and the GelMA/nHA/PLLA membranes demonstrated excellent osteogenic regeneration potential. Therefore, GelMA/nHA/PLLA membranes have wide application prospects in bioengineering applications such as GBR treatment.
纳米纤维聚左旋乳酸（PLLA）膜模拟细胞外基质（ECM）可用于生物医学领域。然而，PLLA的疏水性和较差的骨诱导性能限制了其在引导性骨再生（GBR）中的应用。在这项工作中，首先原位合成甲基丙烯酸化明胶/纳米羟基磷灰石（GelMA/nHA）复合物，然后将其引入PLLA中，通过静电纺丝和光交联制备仿生GelMA/nHA/PLLA膜，模仿ECM的纳米纤维结构和组成。与PLLA和GelMA/PLLA膜相比，新型GelMA/nHA/PLLA膜表现出更好的拉伸、亲水性、吸水性和降解性能。体外生物学评价表明，该膜促进人骨髓间充质干细胞（hBMSC）的增殖、黏附和成骨分化。利用大鼠模型的临界尺寸缺损评估三种膜在体内的骨再生性能，GelMA/nHA/PLLA膜表现出优异的成骨再生潜力。因此，GelMA/nHA/PLLA膜在GBR治疗等生物工程应用中具有广阔的应用前景。
