Department of Mechanical Engineering Fundamentals, Division of Materials Engineering, ATH University of Bielsko-Biala, Willowa 2 street, 43-309, Bielsko-Biała, Poland.
Department of Glass Technology and Amorphous Coatings, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Krakow, Poland.
J Mater Sci Mater Med. 2019 Jun 26;30(7):80. doi: 10.1007/s10856-019-6280-4.
In this work, composite membranes were investigated as future components of a layered implant for the reconstruction of nasal septum. Incorporation of zinc ions into nasal implants could potentially provide antibacterial properties to decrease or eliminate bacterial infections and subsequent surgical complications. Two types of membranes were prepared using an electrospinning method: PCL with bioglass and PCL with bioglass doped with Zn. The aim of this work was to investigate the influence of bioglass addition on the morphology, fiber diameter and composition of the membranes. The apatite-forming ability was examined in Simulated Body Fluid (SBF). The cytotoxicity of the membranes, ALP activity and in vitro mineralization were evaluated in cell culture. The mineralization and ALP activity was higher for polycaprolactone membranes modified with Zn doped bioglass than compared to pure PCL membranes or control material. The results proved that the presence of Zn in the electrospun membranes = influence the osteogenic differentiation of cells.
在这项工作中，复合膜被研究作为分层植入物未来的组成部分，用于重建鼻中隔。将锌离子掺入鼻植入物中可能提供抗菌性能，以减少或消除细菌感染和随后的手术并发症。使用静电纺丝法制备了两种类型的膜：掺有生物玻璃的聚己内酯和掺有 Zn 的生物玻璃掺杂的聚己内酯。本工作旨在研究生物玻璃的添加对膜的形态、纤维直径和组成的影响。在模拟体液（SBF）中研究了磷灰石形成能力。通过细胞培养评估了膜的细胞毒性、碱性磷酸酶（ALP）活性和体外矿化。与纯聚己内酯膜或对照材料相比，用掺 Zn 的生物玻璃改性的聚己内酯膜的矿化和 ALP 活性更高。结果证明，电纺膜中 Zn 的存在会影响细胞的成骨分化。
