Nunes Rodolfo de Melo, Girão Virgínia Claudia Carneiro, Cunha Pablyana Leila Rodrigues, Feitosa Judith Pessoa Andrade, Pinto Ana Carolina Matias Dinelly, Rocha Francisco Airton Castro
Department of Internal Medicine, Faculdade de Medicina da Universidade Federal do Ceará, Fortaleza, Brazil.
Department of Morphology, Faculdade de Medicina da Universidade Federal do Ceará, Fortaleza, Brazil.
Front Med (Lausanne). 2021 Apr 29;8:612370. doi: 10.3389/fmed.2021.612370. eCollection 2021.
We aimed to determine the characteristics that distinguish glycosaminoglycans (GAGs) from osteoarthritis (OA) and normal cartilage and from men and women. Cartilage samples from 30 patients subjected to total joint arthroplasty secondary to OA or fracture (control) were evaluated, and the GAG content (μg/mg dry cartilage) after proteolysis was determined by densitometry, using agarose-gel electrophoresis. Relative percentages of carbon (C), nitrogen (N), and sulfur (S) in GAGs were determined by elemental microanalysis, as well as the zeta potential. Seventeen samples (56.6%) were from patients >70 years old, with 20 (66.6%) from women, and most [20 (66.6%)] were from the hip. The GAG content was similar regardless of patients being >/≤ 70 years old with 96.5 ± 63.5 and 78.5 ± 38.5 μg/mg ( = 0.1917), respectively. GAG content was higher in women as compared to men, with 89.5 ± 34.3 and 51.8 ± 13.3 μg/mg, respectively ( = 0.0022), as well as in OA than fracture samples, with 98.4 ± 63.5 and 63.6 ± 19.6 μg/mg, respectively ( = 0.0355). The GAG extracted from the cartilage of patients >70 years old had increase in N, and there were no gender differences regarding GAG elemental analysis. GAG from OA had a highly significant ( = 0.0005) decrease in S% (1.79% ± 0.25%), as compared to fracture samples (2.3% ± 0.19%), with an associated and significant ( = 0.0001) reduction of the zeta potential in the OA group. This is the first report of a reduced S content in GAG from OA patients, which is associated with a reduced zeta potential.
我们旨在确定可区分骨关节炎（OA）和正常软骨以及男性和女性的糖胺聚糖（GAG）的特征。对30例因OA或骨折（对照）而接受全关节置换术患者的软骨样本进行评估，使用琼脂糖凝胶电泳通过光密度测定法确定蛋白水解后的GAG含量（μg/毫克干软骨）。通过元素微量分析以及zeta电位测定GAG中碳（C）、氮（N）和硫（S）的相对百分比。17个样本（56.6%）来自70岁以上患者，20个（66.6%）来自女性，且大多数[20个（66.6%）]来自髋关节。无论患者年龄> /≤70岁，GAG含量相似，分别为96.5±63.5和78.5±38.5μg/毫克（P = 0.1917）。女性的GAG含量高于男性，分别为89.5±34.3和51.8±13.3μg/毫克（P = 0.0022），且OA样本中的GAG含量高于骨折样本，分别为98.4±63.5和63.6±19.6μg/毫克（P = 0.0355）。从70岁以上患者软骨中提取的GAG的N含量增加，并且在GAG元素分析方面不存在性别差异。与骨折样本（2.3%±0.19%）相比，OA样本的GAG的S%显著降低（P = 0.0005）（1.79%±0.25%），且OA组的zeta电位也有显著降低（P = 0.0001）。这是关于OA患者GAG中S含量降低且与zeta电位降低相关的首份报告。
