Lu Yi, Kong Xianhe, Zhong Weijie, Hu Minhui, Li Chujun
Department of Gastrointestinal Endoscopy, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
Front Mol Biosci. 2021 Apr 28;8:647095. doi: 10.3389/fmolb.2021.647095. eCollection 2021.
Gastric cancer (GC) is the fifth leading cancer in the world. The dysregulated expressions of the thrombospondin (THBS) family were reported to associate with GC, but their relations with tumor stage, prognosis, and correlations with tumor immunity have not been systematically reported. We used versatile public databases such as Oncomine, GEPIA, UALCAN, Kaplan-Meier Plotter, LinkedOmics, STRING, cBioPortal, TIMER, and TISIDB to analyze the expression and mutations of different in GC, along with their functional networks, survival analysis, and tumor-immune interactions. The mRNA levels of , and were significantly higher in the tumor tissues; the expression levels of , , and were higher in stages 2-4 than that of stage 1; patients with high expression of , , , and had poor OS; the genes correlated with were enriched in focal adhesion, glycosaminoglycan biosynthesis, ECM-receptor interaction, and hedgehog signaling pathway; and expression had significant correlations with tumor purity, and all the expression correlated with macrophage and dendritic cells infiltration. THBS2, THBS4, and COMP were potentially diagnostic markers for GC; THBS1, THBS2, THBS4, and COMP were potentially prognostic markers for GC; investigating the relations of THBSs and tumor immunology might help in immunotherapy of GC, while more studies are needed to confirm these results.
胃癌（GC）是全球第五大常见癌症。据报道，血小板反应蛋白（THBS）家族的表达失调与胃癌相关，但其与肿瘤分期、预后的关系以及与肿瘤免疫的相关性尚未得到系统报道。我们使用了Oncomine、GEPIA、UALCAN、Kaplan-Meier Plotter、LinkedOmics、STRING、cBioPortal、TIMER和TISIDB等多种公共数据库，分析了胃癌中不同THBS的表达和突变情况，以及它们的功能网络、生存分析和肿瘤免疫相互作用。THBS1、THBS2和COMP的mRNA水平在肿瘤组织中显著升高；THBS1、THBS2和COMP的表达水平在2-4期高于1期；THBS1、THBS2、THBS4和COMP高表达的患者总生存期较差；与THBS2相关的基因在粘着斑、糖胺聚糖生物合成、细胞外基质-受体相互作用和刺猬信号通路中富集；THBS1和THBS2的表达与肿瘤纯度显著相关，所有THBS的表达均与巨噬细胞和树突状细胞浸润相关。THBS2、THBS4和COMP可能是胃癌的诊断标志物；THBS1、THBS2、THBS4和COMP可能是胃癌的预后标志物；研究THBS与肿瘤免疫学的关系可能有助于胃癌的免疫治疗，不过还需要更多研究来证实这些结果。
