Wang Li, Cao Shan, Zhai Rujun, Zhao Yang, Song Guodong
Department of Gastrointestinal Surgery, The Second Hospital of Tianjin Medical University, Tianjin, China.
Department of Respiratory, The Second Hospital of Tianjin Medical University, Tianjin, China.
Front Genet. 2022 Jan 20;12:760534. doi: 10.3389/fgene.2021.760534. eCollection 2021.
Gastric cancer (GC) remains the fifth most commonly diagnosed malignancy worldwide, with a poor prognosis. The lysyl oxidase (LOX) family, a type of secreted copper-dependent amine oxidases, is comprised of LOX and four LOX-like (LOXL) 1-4 isoforms and has been reported to be dysregulated in a number of different type cancers. However, the diverse expression patterns and prognostic values of LOX family in GC have yet to be systematically analyzed. ONCOMINE, GEPIA, UALCAN, Kaplan-Meier Plotter, LOGpc, cBioPortal, GeneMANIA and Metascape databases were utilized in this study to analyze the expression, prognostic values, mutations and functional networks of LOX family in GC. The mRNA expression levels of LOX, LOXL1 and LOXL2 were significantly higher in GC, the expression level of LOXL3 was contrary in different databases, while the expression level of LOXL4 made no difference; the expression levels of LOX, LOXL1 and LOXL3 were higher in stages 2-4 than that of normal tissues and stage 1, while the mRNA level of LOXL2 in stage 1-4 was higher than normal tissues; patients with high expression of LOX and LOXL 2-4 had poor OS; the genes correlated with LOX and LOXL2 were enriched in extracellular matrix organization, vasculature development and skeletal system development. Our results indicated that the LOX family, especially LOX and LOXL2, might play an important role in GC oncogenesis, and they may become biomarkers for predicting tumor prognosis and potential targets for tumor therapy.
胃癌（GC）仍然是全球第五大最常被诊断出的恶性肿瘤，预后较差。赖氨酰氧化酶（LOX）家族是一种分泌型铜依赖性胺氧化酶，由LOX和四种类LOX（LOXL）1-4亚型组成，据报道在许多不同类型的癌症中存在失调。然而，LOX家族在GC中的多种表达模式和预后价值尚未得到系统分析。本研究利用ONCOMINE、GEPIA、UALCAN、Kaplan-Meier Plotter、LOGpc、cBioPortal、GeneMANIA和Metascape数据库来分析GC中LOX家族的表达、预后价值、突变和功能网络。LOX、LOXL1和LOXL2的mRNA表达水平在GC中显著更高，LOXL3的表达水平在不同数据库中相反，而LOXL4的表达水平没有差异；LOX、LOXL1和LOXL3在2-4期的表达水平高于正常组织和1期，而LOXL2在1-4期的mRNA水平高于正常组织；LOX和LOXL 2-4高表达的患者总生存期较差；与LOX和LOXL2相关的基因在细胞外基质组织、血管发育和骨骼系统发育中富集。我们的结果表明，LOX家族，尤其是LOX和LOXL2，可能在GC的肿瘤发生中起重要作用，它们可能成为预测肿瘤预后的生物标志物和肿瘤治疗的潜在靶点。
