Guarneri Flavia, Tresoldi Enrico Tommaso, Sarli Giuseppe, Boniotti Maria Beatrice, Lelli Davide, Barbieri Ilaria, Bacci Barbara, D'Annunzio Giulia, Amadori Massimo
Laboratory of Animal Welfare, Clinical Chemistry and Veterinary Immunology, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, via A. Bianchi 9, 25124 Brescia, Italy.
DVM, Specialist in Science and Medicine of Laboratory Animals, Italy.
Data Brief. 2021 Feb 22;35:106906. doi: 10.1016/j.dib.2021.106906. eCollection 2021 Apr.
A whole virus, inactivated, Porcine Circovirus 2b (PCV2b) vaccine was submitted to a quantal assay of potency, as explained in detail in our companion paper [1]. To this purpose, twenty, 45-day old piglets, checked for maternally-derived antibody (MDA), were allocated to four groups of 5 animals each; these were vaccinated with 800/266/88/0 nanograms, respectively, of an inactivated PCV2b strain, consisting of two distinct virion populations. Twenty-six days later, all the pigs were challenged intranasally with the homologous PCV2b strain. In the presence of a clear dose-dependent protection in terms of viremia, no such effect was observed in terms of weight gain after challenge. The 800 and 266-ng payloads were associated with neutralizing antibody titers above the MDA levels in oral fluids. Higher levels of viremia in control and 88-ng groups [1] coincided with a higher Natural Killer activity of tracheobronchial lymph node cells from PCV2-infected pigs. The PCV2 ORF2-specific ELISPOT assay for IFN-g- secreting cells showed very few (2-4) ORF2-specific cells/10 peripheral blood mononuclear cells beyond the basal levels under our experimental conditions (non-significant differences among groups). Also, no significant differences were observed in the degree of lymphoid tissue hyperplasia among the different groups.
如我们的配套论文[1]中详细解释的那样，一种全病毒灭活猪圆环病毒2b（PCV2b）疫苗接受了效价的定量测定。为此，对20头45日龄的仔猪进行母源抗体（MDA）检测，并将其分为4组，每组5头动物；分别用800/266/88/0纳克的灭活PCV2b毒株进行接种，该毒株由两个不同的病毒粒子群体组成。26天后，所有猪经鼻内接种同源PCV2b毒株。就病毒血症而言存在明显的剂量依赖性保护作用，但在攻毒后的体重增加方面未观察到此类效果。800纳克和266纳克剂量的疫苗接种后，口腔液中的中和抗体滴度高于母源抗体水平。对照组和88纳克剂量组中较高水平的病毒血症[1]与PCV2感染猪的气管支气管淋巴结细胞较高的自然杀伤活性相一致。在我们的实验条件下，针对分泌IFN-γ的细胞进行的PCV2 ORF2特异性ELISPOT检测显示，除基础水平外，每10个外周血单个核细胞中只有很少（2 - 4个）的ORF2特异性细胞（组间无显著差异）。此外，不同组之间在淋巴组织增生程度上未观察到显著差异。
