Vordermeier Martin, Whelan Adam O
Department of Bacteriology, TB Research Group, Veterinary Laboratories Agency, WeybridgeAddlestone, Surrey, UK.
Methods Mol Biol. 2012;792:219-27. doi: 10.1007/978-1-61779-325-7_17.
Enumeration of antigen-specific cells after vaccination is one of the prime immunological parameters determined when developing vaccines. Due to their exquisite sensitivity (limits of detection can be below 1/100,000 cells), ELISPOT assays are therefore an important tool in vaccine development programs. This is particularly the case for vaccines against diseases that require protective cell-mediated immunity, such as tuberculosis. This chapter describes ELISPOT assays detecting bovine IFN-γ.
疫苗接种后抗原特异性细胞的计数是疫苗研发过程中确定的主要免疫参数之一。由于其极高的灵敏度（检测限可低于1/100,000个细胞），因此酶联免疫斑点（ELISPOT）检测是疫苗研发项目中的一项重要工具。对于针对需要保护性细胞介导免疫的疾病（如结核病）的疫苗而言尤其如此。本章描述了检测牛γ干扰素的ELISPOT检测方法。
