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某生物四个卵巢发育阶段胸神经节转录组的RNA测序数据集 。(你提供的原文不完整,句末缺少关键信息,这是根据已有内容尽量准确翻译的结果)

RNA-Seq dataset of thoracic ganglia transcriptome across four ovarian development stages in .

作者信息

Yodsawat Prasert, Nuanpirom Jiratchaya, Sathapondecha Ponsit, Sangket Unitsa

机构信息

Department of Molecular Biotechnology and Bioinformatics, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand.

Division of Biological Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand.

出版信息

Data Brief. 2021 Apr 20;36:107053. doi: 10.1016/j.dib.2021.107053. eCollection 2021 Jun.

DOI:10.1016/j.dib.2021.107053
PMID:33997198
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8100087/
Abstract

Banana shrimp () is an economically important shrimp in marine aquaculture. Although there is plenty of transcriptome research for this species, the molecular mechanisms in thoracic ganglia of banana shrimp during ovarian maturation have not yet been investigated. Here we report the transcriptomic data of female banana shrimp obtained from thoracic ganglia during ovarian developmental stages. The samples were collected from four stages of ovarian development with two individual shrimps per stage. Total RNA was extracted and used to prepare the sequencing library. Approximately 188 million pair-end raw reads, ranging from 21 to 31 million reads for each library, were generated using an Illumina HiSeq 2500 platform. Quality control was applied to the raw reads before the assembly process. After assembly, the final transcript assembly was generated by vector decontamination, coding regions prediction, redundancy reduction, and foreign sequence depletion. A total of 77,681 transcripts, ranging between 255 and 23,016 bp with an N50 value of 1,167 were obtained to the final assembly. Finally, the final transcripts assembly was evaluated by calculated assembly completeness with Arthropoda orthologous genes dataset. A total of 92.1% of Arthropoda orthologous genes were found in our final assembly. These data might provide benefits for gene discovery, gene annotation, transcript profiling, and other research topics in the context of banana shrimp.

摘要

斑节对虾()是海水养殖中具有重要经济价值的虾类。尽管对该物种已有大量转录组研究,但尚未对斑节对虾卵巢成熟过程中胸神经节的分子机制进行研究。在此,我们报告了在卵巢发育阶段从胸神经节获得的雌性斑节对虾的转录组数据。样本取自卵巢发育的四个阶段,每个阶段有两只对虾。提取总RNA并用于制备测序文库。使用Illumina HiSeq 2500平台生成了约1.88亿对末端原始读数,每个文库的读数范围为2100万至3100万。在组装过程之前对原始读数进行质量控制。组装后,通过载体净化、编码区预测、冗余减少和外源序列去除生成最终转录本组装。最终组装获得了总共77681个转录本,长度在255至23016 bp之间,N50值为1167。最后,使用节肢动物直系同源基因数据集通过计算组装完整性来评估最终转录本组装。在我们的最终组装中发现了总共92.1%的节肢动物直系同源基因。这些数据可能有助于斑节对虾在基因发现、基因注释、转录谱分析及其他研究课题方面的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/523f/8100087/ea4ee6fe3d2d/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/523f/8100087/ea4ee6fe3d2d/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/523f/8100087/ea4ee6fe3d2d/gr1.jpg

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