Center for Genomics and Bioinformatics Research, Division of Biological Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand.
Center for Genomics and Bioinformatics Research, Division of Biological Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand.
Comp Biochem Physiol Part D Genomics Proteomics. 2023 Sep;47:101114. doi: 10.1016/j.cbd.2023.101114. Epub 2023 Jul 29.
Shrimp reproduction is controlled by several factors. Central nervous tissues, especially thoracic ganglia and brain, are known sources of gonad stimulating factors (GSFs) in crustaceans, but the GSFs in shrimp have not yet been clarified. Hence, we aimed to characterize and study putative GSFs from thoracic ganglia of adult female Fenneropenaeus merguiensis. An analysis of thoracic ganglia transcriptome revealed 3224 putative GSFs of a total 77,681 unigenes. Only 376 putative GSFs were differentially expressed during ovarian developmental stages. Eight candidate GSFs were validated for their expression patterns in thoracic ganglia, including the Indian hedgehog gene. F. merguiensis Indian hedgehog (FmIHH) was then investigated for its role in vitellogenesis. The obtained full-length cDNA of FmIHH was similar to other crustacean IHHs rather than Sonic and Desert HHs. The FmIHH was dominantly expressed in thoracic ganglia, and its expression was significantly increased in the vitellogenic stages before being downregulated at the mature stage of ovarian development. Injection of the recombinant FmIHH (His-TF-IHH) protein stimulated vitellogenin expression in ovaries on day 3 and 7, and also increased the gonadosomatic index. In addition, crustacean hyperglycemic hormone expression and total sugar were significantly decreased in eyestalks and hemolymph, respectively, after injection of His-TF-IHH, while lactic acid was increased. Both total sugar and lactic acid were unchanged in ovaries of His-TF-IHH injected shrimp. These results suggested that FmIHH plays a crucial role in vitellogenesis and regulate sugar uptake during ovarian development.
虾类的繁殖受到多种因素的控制。中央神经系统组织，尤其是胸部神经节和脑，是甲壳类动物中性腺刺激因子（GSF）的已知来源，但虾类中的 GSF 尚未得到阐明。因此，我们旨在从成年雌性缅甸虾虎鱼的胸部神经节中鉴定和研究可能的 GSF。胸部神经节转录组分析显示，在总共 77681 个基因中，有 3224 个可能的 GSF。只有 376 个可能的 GSF 在卵巢发育阶段存在差异表达。对 8 个候选 GSF 在胸部神经节中的表达模式进行了验证，其中包括印度 Hedgehog 基因。随后研究了缅甸虾虎鱼的印度 Hedgehog（FmIHH）在卵黄发生中的作用。获得的 FmIHH 全长 cDNA 与其他甲壳类动物的 IHH 相似，而与 Sonic 和 Desert HHs 不同。FmIHH 在胸部神经节中表达丰富，在卵黄发生阶段表达显著增加，然后在卵巢发育的成熟阶段下调。注射重组 FmIHH（His-TF-IHH）蛋白可刺激卵巢中卵黄原蛋白的表达，在第 3 天和第 7 天均有表达，并增加性腺指数。此外，注射 His-TF-IHH 后，眼柄和血淋巴中的甲壳动物高血糖激素表达和总糖分别显著下降，而乳酸增加。注射 His-TF-IHH 后，虾卵巢中的总糖和乳酸均无变化。这些结果表明，FmIHH 在卵黄发生中起关键作用，并调节卵巢发育过程中的糖摄取。
