Szentmihályi Klára, Süle Krisztina, Egresi Anna, Blázovics Anna, May Zoltán
Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, H-1117 Budapest, Magyar tudósok körútja 2, Hungary.
Semmelweis University Institute of Pharmacognosy, H-1026 Budapest, Üllői út 26, Hungary.
Heliyon. 2021 Apr 27;7(4):e06902. doi: 10.1016/j.heliyon.2021.e06902. eCollection 2021 Apr.
Metronidazole has been widely used topically and systemically for more than 50 years but data on its antioxidant properties are still incomplete, unclear and contradictory. Its antioxidant properties are primarily hypothesized based on results, therefore, studies have been performed to determine whether metronidazole has antioxidant activity . We used so-called global spectrophotometric and luminometric methods. Fe/Fe-reducing ability, hydrogen donor activity, hydroxyl radical scavenging property and lipid peroxidation inhibitory activity were investigated. Under the condition used, metronidazole has negligible iron-reducing ability and hydrogen donor activity. The hydroxyl radical scavenging capacity cannot be demonstrated. It acts as a pro-oxidant in the HO/OH-microperoxidase-luminol system, but it can inhibit the induced lipid peroxidation. According to our results, metronidazole has not shown antioxidant activity but can affect redox homeostasis by a ROS-independent mechanism due to its non-direct antioxidant properties.
甲硝唑已在局部和全身广泛使用50多年，但关于其抗氧化特性的数据仍不完整、不明确且相互矛盾。其抗氧化特性主要是基于结果进行推测，因此，已经开展了多项研究以确定甲硝唑是否具有抗氧化活性。我们使用了所谓的全局分光光度法和发光法。研究了铁还原能力、氢供体活性、羟自由基清除特性和脂质过氧化抑制活性。在所使用的条件下，甲硝唑的铁还原能力和氢供体活性可忽略不计。无法证明其具有羟自由基清除能力。在HO/OH-微过氧化物酶-鲁米诺体系中，它作为一种促氧化剂，但可以抑制诱导的脂质过氧化。根据我们的结果，甲硝唑未表现出抗氧化活性，但由于其非直接抗氧化特性，可通过一种不依赖活性氧的机制影响氧化还原稳态。
