Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation, State Research Institute, 24-100 Puławy, Poland.
Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Łódź, 90-236 Łódź, Poland.
Chem Biol Interact. 2017 Jan 25;262:29-37. doi: 10.1016/j.cbi.2016.12.003. Epub 2016 Dec 5.
Taraxacum officinale (dandelion) is a widespread perennial of the Asteraceae family. Dandelion is a rich source of different bioactive compounds, including phenolic compounds, terpenes, carbohydrates, proteins, fatty acids, vitamin and minerals. However, the content of phenolics in tested extracts by various authors was not always well described. Dandelion is also a commonly available food with a long history of human use and as such poses little risk of harm. In this study, we focused on four different phenolic fractions from leaves and petals of dandelion, which might be of great interest. The objective was to investigate the antioxidant properties of the phenolic fractions from dandelion leaves and petals in vitro. Effects of four different phenolic fractions from dandelion leaves and petals on the production of thiobarbituric acid reactive substances (TBARS, a marker of lipid peroxidation) in human plasma were studied in vitro. Their antioxidant properties against human plasma protein carbonylation and oxidation of protein thiols induced by a strong biological oxidant - hydrogen peroxide (HO) or HO/Fe (a donor of hydroxyl radicals) were also examined. The tested fractions of dandelion (0.5-50 μg/mL; the incubation time - 30 min) inhibited plasma lipid peroxidation induced by HO or HO/Fe. However, their antioxidant properties were not concentration-dependent. All tested samples also inhibited plasma protein carbonylation and oxidation of thiol groups in plasma proteins stimulated by oxidants (HO and OH). The obtained results suggest that four tested dandelion fractions, especially phenolic fractions from petals which are recognized as better than leaves source of flavonoids, may be a new and promising source of natural compounds with antioxidant activity beneficial for diseases-associated with oxidative stress, and with changes of hemostasis.
蒲公英（蒲公英）是菊科的一种广泛分布的多年生植物。蒲公英是多种生物活性化合物的丰富来源，包括酚类化合物、萜类化合物、碳水化合物、蛋白质、脂肪酸、维生素和矿物质。然而，不同作者测试的提取物中的酚类含量描述并不总是很好。蒲公英也是一种常见的食品，具有悠久的人类使用历史，因此几乎没有危害的风险。在这项研究中，我们专注于蒲公英叶和花瓣中的四种不同的酚类成分，这可能非常有趣。目的是研究蒲公英叶和花瓣中酚类成分的体外抗氧化性能。研究了蒲公英叶和花瓣的四种不同酚类成分对人血浆中亚油酸丙二醛反应物质（TBARS，脂质过氧化的标志物）生成的影响。还研究了它们对人血浆蛋白羰基化和由强生物氧化剂过氧化氢（HO）或 HO/Fe（羟基自由基供体）诱导的蛋白质巯基氧化的抗氧化性能。蒲公英的测试部分（0.5-50μg/mL；孵育时间-30 分钟）抑制了由 HO 或 HO/Fe 诱导的血浆脂质过氧化。然而，它们的抗氧化性能不是浓度依赖性的。所有测试的样品还抑制了由氧化剂（HO 和 OH）刺激的血浆蛋白羰基化和血浆蛋白中巯基的氧化。研究结果表明，四种测试的蒲公英部分，特别是被认为比叶片更好的黄酮类化合物来源的花瓣酚类部分，可能是一种新的有前途的具有抗氧化活性的天然化合物来源，有益于与氧化应激相关的疾病，以及止血的变化。
