Tang Ting, Tao Faqing, Li Weiqi
School of Life Sciences, Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization, Key Laboratory of Ecological Remediation and Safe Utilization of Heavy Metal-Polluted Soils, Hunan University of Science and Technology, Xiangtan, 411201, China.
Plant Germplasm and Genomics Center, Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China.
Plant Divers. 2020 Jul 25;43(2):163-172. doi: 10.1016/j.pld.2020.07.002. eCollection 2021 Apr.
Manganese (Mn) contamination limits the production and quality of crops, and affects human health by disrupting the food chain. is a pioneer species of Brassicaceae found in mining areas, and has the ability to accumulate heavy metals. However, little is known about the genetic mechanisms of Mn tolerance in . In this study, we found that Mn tolerance and ability to accumulate Mn were higher in than in . The mechanisms underlying the response and recovery of to Mn toxicity were further investigated using transcriptome analysis. A total of 69,862,281 base pair clean reads were assembled into 61,627 high-quality unigenes, of which 41,591 (67.5%) and 39,297 (63.8%) were aligned in the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO), respectively. In response to Mn toxicity, genes were expressed in twelve distinct patterns, which can be divided into four general categories: initial, stable, dose-dependent, and lineage. Genes that were differentially expressed during Mn response and recovery belong to several dominant KEGG pathways. An early response to Mn toxicity in includes the upregulation of genes involved in glutathione metabolism. ATP-binding cassette (ABC) transporter proteins were up-regulated during the entire response phase, and genes involved in glycerophospholipid metabolism were up-regulated during the late phase of the Mn response. Genes in the phenylpropanoid pathway were differentially expressed in the repair process after Mn treatment. These findings reveal ideal material and genetic resources for phytoremediation in Mn-contaminated areas and highlight new knowledge and theoretical perspectives on the mechanisms of Mn tolerance.
锰（Mn）污染限制了农作物的产量和质量，并通过破坏食物链影响人类健康。遏蓝菜属是在矿区发现的十字花科先锋物种，具有重金属积累能力。然而，关于遏蓝菜属对锰耐受性的遗传机制知之甚少。在本研究中，我们发现遏蓝菜属对锰的耐受性和积累锰的能力高于天蓝遏蓝菜。利用转录组分析进一步研究了遏蓝菜属对锰毒性的响应和恢复机制。总共69,862,281个碱基对的 clean reads 被组装成61,627个高质量单基因，其中41,591个（67.5%）和39,297个（63.8%）分别与京都基因与基因组百科全书（KEGG）和基因本体论（GO）比对。响应锰毒性时，基因以十二种不同模式表达，可分为四大类：初始、稳定、剂量依赖性和谱系。在锰响应和恢复过程中差异表达的基因属于几个主要的KEGG途径。遏蓝菜属对锰毒性的早期反应包括参与谷胱甘肽代谢的基因上调。ATP结合盒（ABC）转运蛋白在整个响应阶段上调，参与甘油磷脂代谢的基因在锰响应后期上调。苯丙烷途径中的基因在锰处理后的修复过程中差异表达。这些发现揭示了锰污染地区植物修复的理想材料和遗传资源，并突出了关于锰耐受性机制的新知识和理论观点。
