Dehghan Maryam, Shahbazi Shirin, Salehnia Mojdeh
Anatomy Department, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Medical Genetic Department, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Int J Reprod Biomed. 2021 Apr 22;19(4):361-370. doi: 10.18502/ijrm.v19i4.9062. eCollection 2021 Apr.
Several conflicting results have been reported on the survival and function of transplanted ovaries.
Evaluation of the follicular development and the expression of vascular endothelial growth factor (VEGF) and Bcl-2-associated X protein (BAX) in ovaries transplanted into uni- and bilaterally ovariectomized mice.
In this experimental study, 40 female NMRI mice (21-days-old, 12-15 gr) were ovariectomized uni- and bilaterally (n = 20/ group), while the 8-wk-old mice were considered as intact control group (n = 6). 5 weeks after transplantation at the proestrus stage, the morphology of recovered transplanted ovaries and the proportion of follicles were studied at different developmental stages. The apoptosis cell death by pro-apoptotic protein BAX and the expression of VEGF were evaluated using immunohistochemistry.
In the bilaterally ovariectomized mice, among the 455 counted normal follicles, a lower rate of primordial and primary follicles and a higher rate of preantral and antral follicles were observed (p = 0.002). However, the percentages of preantral and antral follicles, and the corpus luteum were significantly lower in the intact control group (among the 508 counted normal follicles in this group) compared to other transplanted groups (p = 0.002). The number of BAX-positive cells in all groups was not significantly different. The VEGF expression was prominent in vessels of the corpus luteum, and also in the theca layer of large follicles of studied groups.
Early discharge of ovarian reserve was prominent in the bilaterally ovariectomized group but the incidence of apoptotic cells and VEGF expression as angiogenic factor did not differ in both ovariectomized mice. Thus, unilaterally ovariectomy has less side effects on the ovarian reserve compared to bilateral ovariectomy.
关于移植卵巢的存活和功能，已有若干相互矛盾的研究结果报道。
评估移植到单侧和双侧卵巢切除小鼠体内的卵巢中卵泡的发育情况，以及血管内皮生长因子（VEGF）和Bcl-2相关X蛋白（BAX）的表达。
在本实验研究中，40只雌性NMRI小鼠（21日龄，体重12 - 15克）被单侧或双侧卵巢切除（每组n = 20），而8周龄的小鼠作为完整对照组（n = 6）。在发情前期移植5周后，研究回收的移植卵巢的形态以及不同发育阶段卵泡的比例。采用免疫组织化学方法评估促凋亡蛋白BAX导致的凋亡细胞死亡情况以及VEGF的表达。
在双侧卵巢切除的小鼠中，在计数的455个正常卵泡中，原始卵泡和初级卵泡的比例较低，而窦前卵泡和窦卵泡的比例较高（p = 0.002）。然而，与其他移植组相比，完整对照组（该组计数的508个正常卵泡中）的窦前卵泡和窦卵泡以及黄体的百分比显著更低（p = 0.002）。所有组中BAX阳性细胞的数量没有显著差异。VEGF表达在黄体血管以及研究组大卵泡的卵泡膜层中较为突出。
双侧卵巢切除组中卵巢储备的早期释放较为明显，但在双侧卵巢切除的小鼠中，凋亡细胞的发生率以及作为血管生成因子的VEGF表达并无差异。因此，与双侧卵巢切除相比，单侧卵巢切除对卵巢储备的副作用更小。
