Department of Physiological Sciences, Institute of Biological Sciences, University of Brasilia, Darcy Ribeiro Campus, Brasilia, DF, 70910-900, Brazil.
Faculty of Agronomy and Veterinary Medicine, University of Brasilia, Brasilia, DF, Brazil.
Cell Tissue Bank. 2020 Jun;21(2):303-312. doi: 10.1007/s10561-020-09823-6. Epub 2020 Mar 11.
The present study evaluated revascularization time of fresh and cryopreserved cat ovarian tissue after transplantation to subcutaneous tissue. Ovaries of five cats were used and eight pieces of ovarian tissue were taken from each pair of ovaries. Immediately after removal, three pieces were transplanted and one fixed for fresh control. The remaining four pieces were cryopreserved and, after thawing, one was fixed for cryopreservation control and three were transplanted. Grafts were recovered on days 2 (D2), 4 (D4) and 6 (D6) post-transplantation. Blood vessels were identified by immunohistochemistry and doppler ultrasound. Immunohistochemistry showed that the percentages of total tissue area occupied by blood vessels were similar (P > 0.05) in fresh and cryopreserved tissues. In both cases, blood vessel area was significantly higher (P < 0.05) on D4 and D6 compared to D0. Ultrasound analysis showed vascularization improvement on the periphery of grafts from D2 to D4 and from D4 to D6, both in fresh and cryopreserved tissue samples. Nonetheless, there was a significant decrease (P < 0.05) in the percentage of morphologically normal follicles (MNF) after transplantation compared to non-transplanted tissue (D0), both for fresh and cryopreserved samples. Moreover, the number of follicles found in samples was considerably smaller after grafting. In conclusion, revascularization of ovarian tissue autotransplanted to subcutaneous tissue in domestic cats occurs within 4 days after transplantation, both for fresh and cryopreserved tissue. However, large follicular loss has been observed in the first days post-transplantation, especially in cryopreserved tissues.
本研究评估了新鲜和冷冻保存的猫卵巢组织在移植到皮下组织后的再血管化时间。使用了五只猫的卵巢，每对卵巢取出 8 块卵巢组织。取出后立即移植 3 块，1 块用于新鲜对照。其余 4 块进行冷冻保存，解冻后 1 块用于冷冻保存对照，3 块用于移植。移植后第 2（D2）、4（D4）和 6（D6）天回收移植物。通过免疫组织化学和多普勒超声鉴定血管。免疫组织化学显示新鲜和冷冻保存组织中血管总面积的百分比相似（P > 0.05）。在两种情况下，与 D0 相比，D4 和 D6 时血管面积显著更高（P < 0.05）。超声分析显示，在新鲜和冷冻保存组织样本中，从 D2 到 D4 以及从 D4 到 D6 ，移植物周围的血管化均有所改善。尽管如此，与未移植组织（D0）相比，移植后新鲜和冷冻保存组织样本中形态正常卵泡（MNF）的百分比均显著降低（P < 0.05）。此外，移植后样本中发现的卵泡数量明显减少。总之，在猫中，将卵巢组织自体移植到皮下组织后，再血管化在移植后 4 天内发生，新鲜和冷冻保存组织均如此。然而，在移植后的头几天观察到大量卵泡丢失，尤其是在冷冻保存的组织中。
