Hughes Lewis, Roberts Wayne, Johnson Donna
Biomedical Sciences, Leeds Beckett University, Leeds, UK.
Access Microbiol. 2020 Nov 18;3(1):acmi000178. doi: 10.1099/acmi.0.000178. eCollection 2021.
DNA adenine methyltransferase () has been well documented for its role in regulation of replication, mismatch repair and transposition. Recent studies have also suggested a role for in protection against antibiotic stress, although this is not yet fully defined. We therefore evaluated the role of in the development of antibiotic resistance and triclosan-associated cross-resistance.
A significant impact on growth rate was seen in the knockout compared to the parental strain. Known triclosan resistance-associated mutations in were seen regardless of status, with an additional mutation in seen in the knockout. The expression of multiple antibiotic resistance-associated genes was significantly different between the parent and knockout post-resistance induction. Reversion rate assays showed that resistance mechanisms were stable.
knockout had a significant effect on growth, but its role in the development of antibiotic resistance is likely confined to those antibiotics using -containing efflux pumps.
DNA腺嘌呤甲基转移酶（）在复制调控、错配修复和转座中的作用已有充分记录。最近的研究也表明其在抵抗抗生素应激方面发挥作用，尽管这一点尚未完全明确。因此，我们评估了其在抗生素耐药性和三氯生相关交叉耐药性发展中的作用。
与亲本菌株相比，敲除菌株的生长速率受到显著影响。无论菌株状态如何，均发现了已知的与三氯生耐药相关的突变，在敲除菌株中还发现了另外一个突变。在诱导耐药后，亲本菌株和敲除菌株之间多个抗生素耐药相关基因的表达存在显著差异。回复率试验表明耐药机制是稳定的。
敲除对生长有显著影响，但它在抗生素耐药性发展中的作用可能仅限于那些使用含流出泵的抗生素。
