Indian Council of Medical Research-National Institute of Virology, Pune, Maharashtra 411021, India.
STAR Protoc. 2021 Jun 18;2(2):100573. doi: 10.1016/j.xpro.2021.100573. Epub 2021 May 10.
This protocol describes an indirect enzyme-linked immunosorbent assay for qualitative detection of IgG antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Syrian hamster serum samples. We describe the preparation of inactivated virus antigens and the negative control antigen and the use of antigen-coated microtiter plates to detect SARS-CoV-2-specific antibodies from SARS-CoV-2-infected hamsters, including the criteria for differentiating positive versus negative reaction. The limited batch-to-batch variability of this assay has been verified with two batches of independently prepared antigens. For complete details on the use and execution of this protocol, please refer to Mohandas et al. (2021).
本方案描述了一种间接酶联免疫吸附试验，用于定性检测叙利亚仓鼠血清样本中针对严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）的 IgG 抗体。我们描述了灭活病毒抗原和阴性对照抗原的制备，以及使用包被抗原的微量滴定板来检测来自 SARS-CoV-2 感染仓鼠的 SARS-CoV-2 特异性抗体，包括区分阳性与阴性反应的标准。该试验的批间变异性有限，已通过两批独立制备的抗原得到验证。如需了解本方案的详细使用和执行信息，请参考 Mohandas 等人（2021 年）。
