一种使用分离的小鼠肌纤维定量卫星细胞激活的检测方法。

An assay to quantify satellite cell activation using isolated mouse myofibers.

机构信息

Department of Clinical Genetics, Erasmus MC University Medical Center, 3015 GD Rotterdam, the Netherlands.

Department of Pediatrics, Erasmus MC University Medical Center, 3015 GD Rotterdam, the Netherlands.

出版信息

STAR Protoc. 2021 Apr 23;2(2):100482. doi: 10.1016/j.xpro.2021.100482. eCollection 2021 Jun 18.

DOI:10.1016/j.xpro.2021.100482

PMID:33997810

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8095053/

Abstract

Isolated myofibers offer the possibility of study of satellite cells in their niche. We describe a mouse myofiber isolation assay to assess satellite cell activation by quantifying myofiber-derived satellite cell progeny. The assay allows isolation of myofibers from a mouse using standard equipment and reagents. It can be used to compare satellite cells across different mouse models or to evaluate their response to treatments, offering a valuable complementary tool for experimentation.

摘要

孤立的肌纤维为研究卫星细胞在其龛位中的情况提供了可能。我们描述了一种小鼠肌纤维分离测定法，通过定量肌纤维衍生的卫星细胞后代来评估卫星细胞的激活。该测定法使用标准设备和试剂从小鼠中分离肌纤维。它可用于比较不同小鼠模型中的卫星细胞，或评估它们对治疗的反应，为实验提供了一种有价值的补充工具。