Graduate School of Integrated Sciences for Life, Hiroshima University, 1-4-4 Kagamiyama, Higashi, Hiroshima 739-8528, Japan.
Nutrients. 2021 Dec 17;13(12):4531. doi: 10.3390/nu13124531.
Emerging research in human studies suggests an association among vitamin B6, sarcopenia, and muscle strength. However, very little is known regarding its potential role at the cellular level, especially in muscle satellite cells. Therefore, to determine whether vitamin B6 affects the satellite cells, we isolated single myofibers from muscles of vitamin B6-deficient and vitamin B6-supplemented mice. Subsequently, we subjected them to single myofiber culture and observed the number and function of the satellite cells, which remained in their niche on the myofibers. Prior to culture, the vitamin B6-deficient myofibers exhibited a significantly lower number of quiescent satellite cells, as compared to that in the vitamin B6-supplemented myofibers, thereby suggesting that vitamin B6 deficiency induces a decline in the quiescent satellite cell pool in mouse muscles. After 48 and 72 h of culture, the number of proliferating satellite cells per cluster was similar between the vitamin B6-deficient and -supplemented myofibers, but their numbers decreased significantly after culturing the myofibers in vitamin B6-free medium. After 72 h of culture, the number of self-renewing satellite cells per cluster was significantly lower in the vitamin B6-deficient myofibers, and the vitamin B6-free medium further decreased this number. In conclusion, vitamin B6 deficiency appears to reduce the number of quiescent satellite cells and suppress the proliferation and self-renewal of satellite cells during myogenesis.
在人体研究中出现的新研究表明，维生素 B6、肌肉减少症和肌肉力量之间存在关联。然而，关于其在细胞水平上的潜在作用，特别是在肌肉卫星细胞中，人们知之甚少。因此，为了确定维生素 B6 是否会影响卫星细胞，我们从维生素 B6 缺乏和补充的小鼠的肌肉中分离出单个肌纤维。随后，我们将它们进行单细胞纤维培养，并观察卫星细胞的数量和功能，这些卫星细胞仍然保留在肌纤维的龛位上。在培养之前，与维生素 B6 补充的肌纤维相比，维生素 B6 缺乏的肌纤维中静止的卫星细胞数量明显减少，这表明维生素 B6 缺乏会导致小鼠肌肉中静止的卫星细胞池减少。培养 48 和 72 小时后，每个簇的增殖卫星细胞数量在维生素 B6 缺乏和补充的肌纤维之间相似，但在无维生素 B6 的培养基中培养肌纤维后，其数量显著减少。培养 72 小时后，每个簇的自我更新卫星细胞数量在维生素 B6 缺乏的肌纤维中明显较低，无维生素 B6 的培养基进一步降低了这个数量。总之，维生素 B6 缺乏似乎会减少静止的卫星细胞数量，并抑制卫星细胞在肌发生过程中的增殖和自我更新。
