Department of Medical Genetics, Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge, CB2 0XY, UK.
UK Dementia Research Institute, Cambridge, UK.
STAR Protoc. 2021 Apr 27;2(2):100500. doi: 10.1016/j.xpro.2021.100500. eCollection 2021 Jun 18.
We present a protocol for in vivo siRNA-mediated knockdown of a gene of interest in mouse liver using systemic delivery via intravenous injection. We describe a step-by-step protocol for delivery of siRNA particles, with tips on how to optimize dosage. We detail steps for feeding/starving cycles as well as for liver tissue isolation, followed by gene expression analysis, measured at the mRNA and protein levels. For complete information on the generation and use of this protocol, please refer to Wrobel et al. (2020).
我们提出了一种使用静脉注射全身递送在小鼠肝脏中进行感兴趣的基因的体内 siRNA 介导敲低的方案。我们描述了递送 siRNA 颗粒的分步方案，并提供了优化剂量的技巧。我们详细说明了喂养/禁食循环以及肝组织分离的步骤，然后进行基因表达分析，分别在 mRNA 和蛋白质水平上进行测量。有关此方案的生成和使用的完整信息，请参阅 Wrobel 等人（2020 年）。
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