Lu Yuan, Li Na, Zhu Xiaoqin, Pan Jie, Wang Yonglin, Lan Yanyu, Li Yongjun, Wang Aimin, Sun Jia, Liu Chunhua
State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Provincial Key Laboratory of Pharmaceutics, Guizhou Medical University, Guiyang, China.
School of Pharmacy, Guizhou Medical University, Guiyang, China.
Biomed Chromatogr. 2021 Oct;35(10):e5174. doi: 10.1002/bmc.5174. Epub 2021 May 24.
Polygonum orientale L. is a traditional Chinese medicine having extensive pharmacological activities including antimyocardial ischemia (MI) injury properties. Isoorientin, orientin, vitexin, quercitrin, astragalin and protocatechuic acid are the main compounds in P. orientale extract. The aim of this study was to establish an ultra-performance liquid chromatography-tandem mass spectrometry method for the determination of the content of these compounds in urine, feces and bile samples simultaneously and application of the method in a comparative excretion study in normal and MI model rats after oral administration of P. orientale extract. Chromatographic seperation was conducted on an Agilent Eclipse Plus C column with the mobile phase consisting of 0.1% formic acid-acetonitrile and 0.1% formic acid-water. Negative ion multiple reaction monitoring mode was used for quantification. The six compounds had good linearity (r ≥ 0.9921) and acceptable accuracy ranging from 10.10 to -5.82% The relative standard deviations of within-day precision and inter-day precision were <10.45 and 13.44%, respectively. The extraction recovery of the six analytes ranged from 80.31 to 101.47% and the matrix effect was 82.56-102.88%, indicating that the preparations of sample collected form urine, feces and bile were stable throughout analysis. The excretion amount of the six analytes increased in both normal and MI model rats' urine, feces and bile in a 24 h period and became stable between 36 and 48 h after administration. The total excretion rate of six compounds was <5% in urine, feces and bile of normal and MI model rats. The excretion peak period for all compounds in MI rats was slower than that in normal rats. This excretion study provides insights for further application and research on P. orientale.
红蓼是一种具有广泛药理活性的传统中药，包括抗心肌缺血（MI）损伤特性。异荭草素、荭草素、牡荆素、槲皮苷、紫云英苷和原儿茶酸是红蓼提取物中的主要化合物。本研究的目的是建立一种超高效液相色谱 - 串联质谱法，用于同时测定尿液、粪便和胆汁样品中这些化合物的含量，并将该方法应用于正常大鼠和MI模型大鼠口服红蓼提取物后的比较排泄研究。色谱分离在安捷伦Eclipse Plus C柱上进行，流动相由0.1%甲酸 - 乙腈和0.1%甲酸 - 水组成。采用负离子多反应监测模式进行定量。这六种化合物具有良好的线性关系（r≥0.9921），准确度在10.10%至 -5.82%之间，日内精密度和日间精密度的相对标准偏差分别<10.45%和13.44%。六种分析物的提取回收率在80.31%至101.47%之间，基质效应为82.56% - 102.88%，表明从尿液、粪便和胆汁中采集的样品制剂在整个分析过程中是稳定的。在24小时内，正常大鼠和MI模型大鼠的尿液、粪便和胆汁中六种分析物的排泄量均增加，并在给药后36至48小时之间趋于稳定。正常大鼠和MI模型大鼠尿液、粪便和胆汁中六种化合物的总排泄率<5%。MI大鼠中所有化合物的排泄高峰期均比正常大鼠慢。该排泄研究为红蓼的进一步应用和研究提供了见解。
