Totou Nádia Lúcia, de Moura Samara Silva, Martins Júnior Francisco de Assis Dias, de Sousa Frederico Barros, Coelho Daniel Barbosa, de Oliveira Emerson Cruz, Dos Santos Robson Augusto Souza, Becker Lenice Kappes, de Lima Wanderson Geraldo
Biological Sciences Research Center - Postgraduate Program in Biological Sciences, Federal University of Ouro Preto, Ouro Preto, Minas Gerais, Brazil.
School of Physical Education, Federal University of Ouro Preto, Ouro Preto, Minas Gerais, Brazil.
Exp Physiol. 2021 Aug;106(8):1710-1719. doi: 10.1113/EP089308. Epub 2021 Jun 26.
What is the central question of this study? Eccentric contraction exercises cause damage to muscle fibres and induce inflammatory responses. The exacerbation of this process can induce deposition of fibrous connective tissue, leading to decreased muscle function. The aim of this study was to examine the role of angiotensin-(1-7) in this context. What is the main finding and its importance? Our results show that oral treatment with angiotensin-(1-7) decreases muscle damage induced by eccentric exercise, reducing inflammation and fibrosis in the gastrocnemius and soleus muscles. This study shows a potential effect of angiotensin-(1-7) for the prevention of muscle injuries induced by physical exercise.
Eccentric contraction exercises cause damage to the muscle fibres and induce an inflammatory reaction. The protective effect of angiotensin-(1-7) [Ang-(1-7)] in skeletal muscle has led us to examine the role of this peptide in modifying processes associated with inflammation and fibrogenesis induced by eccentric exercise. In this study, we sought to investigate the effects of oral administration of Ang-(1-7) formulated in hydroxypropyl β-cyclodextrin (HPβ-CD) in prevention and treatment of muscle damage after downhill running. Male Wistar rats were divided into three groups: control (untreated and not exercised; n = 10); treated/exercised HPβ-CD Ang-(1-7) (n = 40); and treated/exercised HPβ-CD (n = 40). Exercised groups were subjected to a single eccentric contraction exercise session on a treadmill inclined to -13° at a constant speed of 20 m/min, for 60 min. Oral administration of HPβ-CD Ang-(1-7) and HPβ-CD was performed 3 h before the exercise protocol and daily as a single dose, until the end of the experiment. Samples were collected 4, 12, 24, 48 and 72 h after the exercise session. The animals treated with the Ang-(1-7) showed lower levels of creatine kinase, lower levels of tumor necrosis factor-α in soleus muscle and increased levels of interleukin-10 cytokines. The inflammatory cells and deposition of fibrous connective tissue in soleus and gastrocnemius muscles were lower in the group treated with Ang-(1-7). The results of this study show that treatment with an oral formulation of Ang-(1-7) enhances the process of repair of muscle injury induced by eccentric exercise.
本研究的核心问题是什么？离心收缩运动会导致肌纤维损伤并引发炎症反应。该过程的加剧会诱导纤维结缔组织沉积，导致肌肉功能下降。本研究的目的是探讨血管紧张素 -（1 - 7）在此背景下的作用。主要发现及其重要性是什么？我们的结果表明，口服血管紧张素 -（1 - 7）可减少离心运动诱导的肌肉损伤，减轻腓肠肌和比目鱼肌的炎症和纤维化。本研究显示了血管紧张素 -（1 - 7）在预防体育锻炼引起的肌肉损伤方面的潜在作用。
离心收缩运动会导致肌纤维损伤并引发炎症反应。血管紧张素 -（1 - 7）[Ang -（1 - 7）]在骨骼肌中的保护作用促使我们研究该肽在调节离心运动诱导的炎症和纤维生成相关过程中的作用。在本研究中，我们试图研究口服羟丙基β - 环糊精（HPβ - CD）配制的Ang -（1 - 7）在预防和治疗下坡跑后肌肉损伤中的作用。雄性Wistar大鼠分为三组：对照组（未治疗且未运动；n = 10）；经HPβ - CD Ang -（1 - 7）治疗/运动组（n = 40）；以及经HPβ - CD治疗/运动组（n = 40）。运动组在倾斜13°的跑步机上以20米/分钟的恒定速度进行单次离心收缩运动，持续60分钟。在运动方案前3小时口服HPβ - CD Ang -（1 - 7）和HPβ - CD，并每天单剂量给药，直至实验结束。在运动后4、12、24、48和72小时采集样本。用Ang -（1 - 7）治疗的动物肌酸激酶水平较低，比目鱼肌中肿瘤坏死因子 -α水平较低，白细胞介素 - 10细胞因子水平升高。用Ang -（1 - 7）治疗的组中比目鱼肌和腓肠肌中的炎症细胞和纤维结缔组织沉积较少。本研究结果表明，口服Ang -（1 - 7）制剂治疗可增强离心运动诱导的肌肉损伤修复过程。
