School of Biomolecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC), Wang Chan Valley, Rayong 21210, Thailand.
Anal Chem. 2021 Jun 1;93(21):7557-7561. doi: 10.1021/acs.analchem.1c00996. Epub 2021 May 17.
A sustainable three-electrode platform for affordable microfluidic electroanalysis is described. The device can be handmade using common tools and, facilitating broad applicability, is indefinitely reusable through simple surface polishing. Compact prototypes with Pt counter, Pt working, and Ag/AgCl reference electrode disks were combined with silicone lid plates containing a microchannel for electrolyte flow. Redox voltammetry/amperometry of excellent quality was achieved in static and flowing ferricyanide solutions, respectively. Modified with a glucose oxidase surface layer, base plate Pt WEs performed very well as amperometric biosensors for microfluidic blood glucose testing. The electrode system is recyclable, compatible with matching lid plate microchannels, and functionally adaptable regarding the constituent metal and electrode surface modifications. This asset combination makes the device a sustainable detection tool for microfluidic electroanalysis, with applications ranging from direct detection of redox-active analytes to bioreceptor-assisted biosensing. It avoids costly microfabrication with clean-room use, and the accessibility of microfluidic EC (bio)sensing is thus greatly increased, especially for users with restricted budgets.
一种用于经济实惠的微流控电分析的可持续三电极平台被描述。该设备可以使用常见的工具手工制作，并且通过简单的表面抛光可以无限期重复使用，从而促进了广泛的适用性。带有 Pt 对电极、Pt 工作电极和 Ag/AgCl 参比电极盘的紧凑型原型与含有电解质流动微通道的硅酮盖片组合在一起。在静态和流动的铁氰化物溶液中分别实现了高质量的氧化还原伏安法/电流法测定。经过葡萄糖氧化酶表面层修饰后，基底 Pt WE 作为微流控血糖仪的电流型生物传感器表现非常出色。该电极系统可回收利用，与匹配的盖片微通道兼容，并且在组成金属和电极表面修饰方面具有功能适应性。这种资产组合使该设备成为微流控电分析的可持续检测工具，其应用范围从直接检测氧化还原活性分析物到生物受体辅助生物传感。它避免了使用洁净室的昂贵微制造，从而大大增加了微流控 EC（生物）传感的可及性，特别是对于预算有限的用户。
