Sakdaphetsiri Kittiya, Teanphonkrang Somjai, Schulte Albert
School of Biomolecular Science and Engineering (BSE), Vidyasirimedhi Institute of Science and Technology, Rayong 21210 Thailand.
ACS Omega. 2022 Jun 3;7(23):19347-19354. doi: 10.1021/acsomega.2c00925. eCollection 2022 Jun 14.
Novel glucose biosensors were constructed by loading glucose oxidase (GO) into the nanopores of homogenous carbon nanotube (CNT) films on the surface of Pt disk electrodes and trapping the enzyme by subsequent deposition of polyacrylic acid (PAA), forming PAA/GO-CNT-modified Pt disks. In amperometric biosensing with anodic hydrogen peroxide (HO) detection at a potential of +600 mV, increasing electrolyte glucose concentrations produced instantaneous steps in the HO oxidation current. Glucose biosensor amperometry was feasible down to 10 μM, with a sensitivity of about 34 μA mM cm and linear current response up to 5 mM. The biosensors reliably determined glucose concentrations in human serum and a beverage. Successful trials with PAA/GO-CNT-modified screen-printed Pt electrode disks demonstrated the potential of this means of enzyme fixation in biosensor mass fabrication, which offers a unique combination of cheap availability of the two matrix constituents and sensor layer formation through simple drop-and-dry steps. PAA/GO-CNT/Pt biosensors are green and user-friendly bioanalytical tools that do not need large budgets, special skills, or laboratory amenities for their production. Any user, from industrial, university, or school laboratories, even if inexperienced in biosensor construction, can prepare functional biosensors with GO, as in these proof-of-principle studies, or with other redox enzymes, for clinical, environmental, pharmaceutical, or food sample analysis.
通过将葡萄糖氧化酶（GO）负载到铂盘电极表面均匀的碳纳米管（CNT）薄膜的纳米孔中，并通过随后沉积聚丙烯酸（PAA）来捕获酶，构建了新型葡萄糖生物传感器，形成了PAA/GO-CNT修饰的铂盘。在阳极过氧化氢（HO）检测电位为+600 mV的安培生物传感中，增加电解质葡萄糖浓度会使HO氧化电流产生瞬时阶跃。葡萄糖生物传感器安培法在低至10 μM时是可行的，灵敏度约为34 μA mM cm，线性电流响应高达5 mM。该生物传感器能够可靠地测定人血清和饮料中的葡萄糖浓度。使用PAA/GO-CNT修饰的丝网印刷铂电极盘进行的成功试验证明了这种酶固定方法在生物传感器大规模制造中的潜力，该方法提供了两种基质成分廉价可得性以及通过简单的滴加和干燥步骤形成传感器层的独特组合。PAA/GO-CNT/Pt生物传感器是绿色且用户友好的生物分析工具，其生产不需要大量预算、特殊技能或实验室设施。任何来自工业、大学或学校实验室的用户，即使在生物传感器构建方面没有经验，也可以像在这些原理验证研究中一样，用GO或其他氧化还原酶制备功能性生物传感器，用于临床、环境、制药或食品样品分析。
