Doillon C J, Dunn M G, Berg R A, Silver F H
Scan Electron Microsc. 1985(Pt 2):897-903.
Collagen fiber diameters, amount of birefringent collagen (brightness) and birefringence retardation were measured in implanted collagen-based sponges containing hyaluronic acid (HA) and fibronectin (FN). In the presence of HA and FN, increased number of fibroblasts and brightness were observed 6 days after wounding. Increased brightness in the presence of HA and FN reflected increased deposition of oriented collagen fibers. From days 9 to 12, increased fiber diameters were similar in implanted collagen-based sponges with or without HA and FN. Increased birefringence retardation in sponges containing HA and FN was consistent with increased packing density of collagen fibers observed by scanning electron microscopy. Our results suggest that HA and FN are effective in promoting fibroblast movement into a collagen sponge and deposition of collagen fibers during the early phases of wound healing. Use of a collagen-based sponge containing HA and FN may enhance collagen deposition in situations where healing is compromised as in the case of dermal ulcers.
在含有透明质酸（HA）和纤连蛋白（FN）的植入型胶原海绵中，测量了胶原纤维直径、双折射胶原量（亮度）和双折射延迟。在有HA和FN存在的情况下，受伤6天后观察到成纤维细胞数量增加且亮度增强。HA和FN存在时亮度增加反映了定向胶原纤维沉积增加。从第9天到第12天，在有或没有HA和FN的植入型胶原海绵中，纤维直径增加情况相似。含有HA和FN的海绵中双折射延迟增加与扫描电子显微镜观察到的胶原纤维堆积密度增加一致。我们的结果表明，HA和FN在伤口愈合早期能有效促进成纤维细胞向胶原海绵移动以及胶原纤维沉积。在愈合受损的情况下，如皮肤溃疡，使用含有HA和FN的胶原海绵可能会增强胶原沉积。
