Ahmad Ajaz, Zafar Atif, Zargar Seema, Bazgaifan Arwa, Wani Tanveer A, Ahmad Masood
Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh, India.
Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
J Biomol Struct Dyn. 2022;40(19):9306-9317. doi: 10.1080/07391102.2021.1926325. Epub 2021 May 17.
Edifenphos (EDF) is an organophosphorus pesticide with antifungal and anti-insecticidal properties. However, EDF accumulates in various agricultural products and causes potential hazards to human health. Although numerous reports have indicated EDF accumulation in agricultural products, toxic effects on cellular system is poorly understood. In the present study, we investigated the cytotoxicity and genotoxicity of EDF in rat hepatocytes and its amelioration by apigenin (a dietary flavonoid). Results showed that EDF inhibited the cell viability, induced oxidative stress, DNA damage, loss of mitochondrial membrane potential (ΔΨm) and caspase-9/-3 activation in rat hepatocytes. Incubation of hepatocytes with N-acetyl cysteine (ROS scavenger) significantly abrogated the ROS generation and apoptosis caused by EDF. In addition, this study also showed that apigenin significantly suppressed the toxic effects of EDF by quenching ROS production thereby abrogating the caspase-9/-3 and apoptosis activation in hepatocytes. Taken together, the findings of this study demonstrate that EDF induces cytotoxicity and DNA damage in hepatocytes, and apigenin can be considered as an effective dietary anti-oxidant regimen against EDF- induced toxicity in cellular system.Communicated by Ramaswamy H. Sarma.
稻瘟净（EDF）是一种具有抗真菌和杀虫特性的有机磷农药。然而，EDF会在各种农产品中蓄积，并对人类健康造成潜在危害。尽管已有大量报道表明EDF在农产品中蓄积，但对其细胞系统毒性作用的了解却很少。在本研究中，我们调查了EDF对大鼠肝细胞的细胞毒性和遗传毒性，以及芹菜素（一种膳食黄酮类化合物）对其的改善作用。结果表明，EDF抑制大鼠肝细胞的细胞活力，诱导氧化应激、DNA损伤、线粒体膜电位（ΔΨm）丧失以及半胱天冬酶-9/-3激活。用N-乙酰半胱氨酸（ROS清除剂）孵育肝细胞可显著消除EDF引起的ROS生成和细胞凋亡。此外，本研究还表明，芹菜素通过淬灭ROS生成，从而消除肝细胞中的半胱天冬酶-9/-3激活和细胞凋亡，显著抑制了EDF的毒性作用。综上所述，本研究结果表明，EDF可诱导肝细胞的细胞毒性和DNA损伤，芹菜素可被视为一种有效的膳食抗氧化方案，以对抗细胞系统中EDF诱导的毒性。由拉马斯瓦米·H·萨尔马通讯。
