Sweety Shahnaz Parvin, Rupok Tahsin Ahmed, Parvin Mst Shahnaj, Barmon Jaytirmoy, Yeasmin Mst Sarmina, Islam Md Ekramul
Department of Pharmacy, University of Rajshahi, Rajshahi 6205, Bangladesh; Department of Pharmacy, Manarat International University, Dhaka 1212, Bangladesh.
Department of Pharmacy, University of Rajshahi, Rajshahi 6205, Bangladesh.
J Genet Eng Biotechnol. 2025 Sep;23(3):100553. doi: 10.1016/j.jgeb.2025.100553. Epub 2025 Aug 14.
Lagerstroemia speciosa is traditionally used for treating diabetes and inflammation; however, its anticancer potential remains unexplored. This study assesses the antioxidant and anticancer activities of L. speciosa bark extract, with a focus on Ehrlich Ascites Carcinoma (EAC) cells in mice. The crude methanol extract (CME) was subdivided into n-hexane (NHF), chloroform (CHF), ethyl acetate (EAF), and aqueous (AQF) fractions. In vitro antioxidant assays identified CHF as the most active fraction, exhibiting high phenolic and flavonoid content. CHF and EAF were further investigated for anticancer activity in an EAC-induced mouse model, where CHF significantly (P < 0.05) reduced tumor cell count compared to EAF. Phytochemical characterization using FTIR and GC-MS revealed bioactive compounds, including 9-Methoxybicyclo[6.1.0]nona-2,4,6-triene and 9,12-Octadecadienoic acid methyl ester (E,E). Molecular docking studies demonstrated strong interactions between these compounds and key cancer-related proteins, p53 and Topoisomerase-II, suggesting potential anticancer mechanisms. Overall, this study shows that L. speciosa bark extract has therapeutic potential, especially for CHF. Bioactive compounds like 9,12-octadecadienoic acid methyl ester (E,E) and 9-methoxybicyclo [6.1.0]nona-2,4,6-triene contribute to the extract's antioxidant and anticancer effects in EAC models, possibly by modulating p53 and Topoisomerase-II.
传统上，紫薇被用于治疗糖尿病和炎症；然而，其抗癌潜力尚未得到探索。本研究评估了紫薇树皮提取物的抗氧化和抗癌活性，重点关注小鼠艾氏腹水癌（EAC）细胞。粗甲醇提取物（CME）被细分为正己烷（NHF）、氯仿（CHF）、乙酸乙酯（EAF）和水相（AQF）馏分。体外抗氧化试验确定CHF为活性最高的馏分，其酚类和黄酮类含量高。在EAC诱导的小鼠模型中，进一步研究了CHF和EAF的抗癌活性，与EAF相比，CHF显著（P<0.05）降低了肿瘤细胞计数。使用傅里叶变换红外光谱（FTIR）和气相色谱-质谱联用（GC-MS）进行的植物化学表征揭示了生物活性化合物，包括9-甲氧基双环[6.1.0]壬-2,4,6-三烯和9,12-十八碳二烯酸甲酯（E,E）。分子对接研究表明这些化合物与关键的癌症相关蛋白p53和拓扑异构酶-II之间存在强相互作用，提示了潜在的抗癌机制。总体而言，本研究表明紫薇树皮提取物具有治疗潜力，尤其是CHF。像9,12-十八碳二烯酸甲酯（E,E）和9-甲氧基双环[6.1.0]壬-2,4,6-三烯这样的生物活性化合物可能通过调节p53和拓扑异构酶-II，对提取物在EAC模型中的抗氧化和抗癌作用有贡献。
