[Determination of free and bound forms of estrogen receptors in rat liver cytosol using a method of ligand turnover at low temperature].

A procedure is described for estimation of free and bound estrogen receptors in rat liver cytosol at low temperature using ligand turnover in presence of sodium thiocyanate. Total content of estrogen receptors was similar in cytosol of intact and ovariectomized rat females but about 30% of estrogen receptors were estimated in cytosol of intact animals as estrogen-receptor complexes. Within 24 hrs after single administration of 500 mg estradiol E2 into ovariectomized rat females approximately 50% of estrogen receptors were shown to be bound with hormone in cytosol.