Implementation of a next-generation sequencing-based targeted approach for full-length gene sequencing.

To evaluate the application of next-generation sequencing-based targeted protocol for full-length gene sequencing analysis. The developed sequencing protocol was applied to analyze human DNA samples (n = 7) obtained from tuberculosis patients admitted to the Riga East University Hospital, Center of Tuberculosis and Lung diseases. The sequencing data quality was sufficient for the detection of already known genetic variants, as well as for identifying rare and novel variants dispersed throughout the gene with a high degree of confidence. Developed protocol can be applied in subpopulation level association studies to determine whether specific genetic variants or variant combinations from multiple regions of the gene are of clinical significance.