RNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, United States.
National Institute of Biological Sciences, Beijing, China.
Elife. 2021 May 18;10:e63300. doi: 10.7554/eLife.63300.
Germlines shape and balance heredity, integrating and regulating information from both parental and foreign sources. Insights into how germlines handle information have come from the study of factors that specify or maintain the germline fate. In early embryos, the CCCH zinc finger protein PIE-1 localizes to the germline where it prevents somatic differentiation programs. Here, we show that PIE-1 also functions in the meiotic ovary where it becomes SUMOylated and engages the small ubiquitin-like modifier (SUMO)-conjugating machinery. Using whole-SUMO-proteome mass spectrometry, we identify HDAC SUMOylation as a target of PIE-1. Our analyses of genetic interactions between and SUMO pathway mutants suggest that PIE-1 engages the SUMO machinery both to preserve the germline fate in the embryo and to promote Argonaute-mediated surveillance in the adult germline.
生殖系塑造和平衡遗传，整合和调节来自父母和外源的信息。对生殖系如何处理信息的深入了解来自于研究特异性或维持生殖系命运的因素。在早期胚胎中，CCCH 锌指蛋白 PIE-1 定位于生殖系，阻止体细胞分化程序。在这里，我们表明 PIE-1 也在减数分裂的卵巢中发挥作用，在那里它被 SUMO 化，并与小泛素样修饰物 (SUMO) 连接酶结合。使用全 SUMO 蛋白组质谱法，我们鉴定了 HDAC SUMOylation 是 PIE-1 的靶标。我们对 和 SUMO 途径突变体之间遗传相互作用的分析表明，PIE-1 既参与 SUMO 机器的运作以在胚胎中保持生殖系命运，又促进成年生殖系中 Argonaute 介导的监视。
