Iida Y, Kasagi K, Tokuda Y, Arai K, Misaki T, Konishi J
Department of Radiology and Nuclear Medicine, Kyoto University School of Medicine, Japan.
Acta Endocrinol (Copenh). 1988 Aug;118(4):580-6. doi: 10.1530/acta.0.1180580.
We studied antibody-dependent mononuclear cell-mediated growth inhibition of thyroid cells in 18 untreated patients with Graves' disease, 18 patients with chronic thyroiditis, and 15 normal subjects by measuring the ability of their sera to inhibit [3H]thymidine incorporation into DNA in a rat thyroid cell line, FRTL-5, in the presence of normal mononuclear cells. [3H]thymidine incorporation was significantly inhibited in the presence of sera from patients with Graves' disease and chronic thyroiditis (P less than 0.001), whereas it was not affected in normal subjects. A significant correlation was observed between the inhibition of [3H]thymidine incorporation and the titre of anti-microsomal antibodies (P less than 0.05). The inhibitory effect on [3H]thymidine incorporation was significantly abolished when serum pre-absorbed with human thyroid membranes was used (P less than 0.005). These inhibitory effects on [3H]thymidine incorporation significantly correlated with those obtained by using IgG fractions (P less than 0.01). These data indicate that antibody-dependent mononuclear cell-mediated growth inhibition may play a role in thyroid cell growth regulation in patients with autoimmune thyroid disease.
我们通过在正常单核细胞存在的情况下，检测18例未经治疗的格雷夫斯病患者、18例慢性甲状腺炎患者和15名正常受试者的血清抑制大鼠甲状腺细胞系FRTL-5中[3H]胸腺嘧啶核苷掺入DNA的能力，研究了抗体依赖性单核细胞介导的甲状腺细胞生长抑制情况。在格雷夫斯病患者和慢性甲状腺炎患者的血清存在时，[3H]胸腺嘧啶核苷掺入受到显著抑制（P<0.001），而在正常受试者中则未受影响。[3H]胸腺嘧啶核苷掺入的抑制与抗微粒体抗体滴度之间存在显著相关性（P<0.05）。当使用用人甲状腺膜预先吸收的血清时，对[3H]胸腺嘧啶核苷掺入的抑制作用显著消除（P<0.005）。这些对[3H]胸腺嘧啶核苷掺入的抑制作用与使用IgG组分获得的抑制作用显著相关（P<0.01）。这些数据表明，抗体依赖性单核细胞介导的生长抑制可能在自身免疫性甲状腺疾病患者的甲状腺细胞生长调节中起作用。
